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Definite spontaneous cell-mediated cytotoxicity and Hnk-1 cells in the human large intestine

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Summary

Spontaneous cell-mediated cytotoxicity (SCMC) and the marker of natural killer (NK) cells mediating SCMC of the human large intestine were studied. Lamina proprial lymphoid cells (LPL) were isolated by sequential dithiothreitol-EDTA-collagenase treatment of the gut specimen. SCMC was measured by the chromium release method. Target cells included P4788 in monolayer, a cell line derived from colon cancer, Chang cells in monolayer, and K562 in suspension. Target cells in monolayer including colon cancer cell line were chosen because they were thought to be more appropriate to assess SCMC for lymphoid cells in the solid organ. While lower compared to cytotoxicities (CT) by peripheral blood lymphoid cells (PBL), define CT were observed in LPL against all three targets. NK cells marker was studied both on LPL by an indirect fluorescent antibody method and on the gut tissue by indirect immunoperoxidase staining using anti HNK-1 monoclonal antibody which defines virtually all NK cells. HNK-1 positive (HNK-1 +) cells were identified in both methods. HNK-1 + cells were observed in the epithelium, lamina propria, and lymph follicle with or without germinal centers. These results clearly demonstrated the presence of SCMC and HNK-1 + cells in the human large bowel.

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Authors and Affiliations

  1. The First Department of Internal Medicine, Akita University School of Medicine, 1-1-1, Hondo, Akita City, 010, Akita, Japan

    Mitsuro Chiba M.D., Hiromasa Ohta M.D. & Osamu Masamune M.D.

  2. First Department of Internal Medicine, Hirosaki University School of Medicine, 036, Hirosaki, Japan

    Yutaka Yoshida M. D.

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  1. Mitsuro Chiba M.D.
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  2. Hiromasa Ohta M.D.
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  3. Osamu Masamune M.D.
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  4. Yutaka Yoshida M. D.
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Chiba, M., Ohta, H., Masamune, O. et al. Definite spontaneous cell-mediated cytotoxicity and Hnk-1 cells in the human large intestine. Gastroenterol Jpn 21, 584–593 (1986). https://doi.org/10.1007/BF02774486

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  • DOI: https://doi.org/10.1007/BF02774486

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