Skip to main content

Advertisement

SpringerLink
Log in

A comparative study of passive hemagglutina tion and immunofluorescence methods for the detection of mitochondrial antibodies in sera from patients with liver diseases

  • Original Article
  • Published:
Gastroenterologia Japonica Aims and scope Submit manuscript

Summary

Serum mitochondrial antibodies were capable of demonstration by the passive hemagglutination method (PHA) using sheep red blood cells coated with mitochondrial inner membrane components. Titers of mitochondrial antibodies obtained by PHA were well correlated with the antibody titers by the immunofluorescence method (IF) and the former method was more sensitive than the latter. Of 15 patients with primary biliary cirrhosis, mitochondrial antibodies were detected by PHA in the sera from 14 patients including two patients with negative results by IF. The antibodies were not detected by PHA in any sera from both 30 patients with chronic hepatitis and 26 with liver cirrhosis. In conclusion, PHA could be an useful diagnostic tool for the detection of serum mitochondrial antibodies in the patients who were suspected of primary biliary cirrhosis in spite of negative results by the conventional IF.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Walker JG, Doniach D., Roitt IM, et al: Serological tests in diagnosis of primary biliary cirrhosis. Lancet 1:827, 1965

    Article  PubMed  CAS  Google Scholar 

  2. Doniach D., Roitt IM, Walker JG, et al: Tissue antibodies in primary biliary cirrhosis, active chronic (lupoid) hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implications. Clin Exp Immunol 1: 237, 1966

    PubMed  CAS  Google Scholar 

  3. Berg PA, Doniach D., Roitt IM: Mitochondrial antibodies in primary biliary cirrhosis. I. Localization of the antigen to mitochondrial membranes. J Exp Med 126:277, 1967

    Article  PubMed  CAS  Google Scholar 

  4. Berg PA, Muscatello U., Home RW, et al: Mitochondrial antibodies in primary biliary cirrhosis. II. The complement fixing antigen as a component of mitochondrial inner membranes. Br J Exp Pathol 50: 200, 1969

    PubMed  CAS  Google Scholar 

  5. Berg PA, Roitt IM, Doniach D., et al: Mitochondrial antibodies in primary biliary cirrhosis. III. Characterization of the inner-membrane complement fixing antigen. Clin Exp Immunol 4: 511, 1969

    PubMed  CAS  Google Scholar 

  6. Berg PA, Roitt IM, Doniach D., et al: Mitochondrial antibodies in primary biliary cirrhosis. IV. Significance of membrane structure for the complement fixing antigen. Immunology 17: 281, 1969

    PubMed  CAS  Google Scholar 

  7. Ben-Yoseph Y., Shapira E., Doniach D.: Further purification of the mitochondrial inner membrane autoantigen reacting with primary biliary cirrhosis sera. Immunology 26: 311, 1974

    PubMed  CAS  Google Scholar 

  8. Rizzetto M., Swana G., Doniach D.: Microsomal antibodies in active chronic hepatitis and other disorders. Clin Exp Immunol 15: 331, 1973

    PubMed  CAS  Google Scholar 

  9. Homberg JC, Rizzeto M., Doniach D.: Ribosomal antibodies detected by immunofluorescence in systemic lupus erythematosus and other collagenoses. Clin Exp Immunol 17: 617, 1974

    PubMed  CAS  Google Scholar 

  10. Rizzetto M., Doniach D.: Types of reticulin antibodies detected in human sera by immunofluorescence. J Clin Pathol 26: 841, 1973

    Article  PubMed  CAS  Google Scholar 

  11. Doniach D, Walker G: Immunopathology of liver disease, in “Progress in Liver Diseases” Vol. 4, by Popper H, Schaffner F. Grune & Stratton, New York, 1972, p 381.

  12. Rubin E., Schaffner F., Popper H.: Primary biliary cirrhosis. Chronic non-suppurative destructive cholangitis. Am J Pathol 46: 387, 1965

    PubMed  CAS  Google Scholar 

  13. Scheuer PJ: Primary biliary cirrhosis. Proc R Soc Med 60: 1257, 1967

    PubMed  CAS  Google Scholar 

  14. Ichida F, Sasaki H, Wakisaka G: Observation on chronic hepatitis in Japan: Morphology, etiology and sequelae, in “Progress in Liver Diseases” Vol. 2, by Popper H, Schaffner F. Grune & Stratton, New York, 1965, p 416

  15. Parsons DF, Williams GR, Chance B.: Characteristics of isolated and purified preparations of the outer and inner membranes of mitochondria. Ann NY Acad Sci 137: 643, 1966

    Article  PubMed  CAS  Google Scholar 

  16. Goudie RB, MacSween RNM, Goldberg DM: Serological and histological diagnosis of primary biliary cirrhosis. J Clin Pathol 19: 527, 1966

    Article  PubMed  CAS  Google Scholar 

  17. Paronetto F., Schaffner F., Popper H.: Antibodies to cytoplasmic antigens in primary biliary cirrhosis and chronic active hepatitis. J Lab Clin Med 69: 979, 1967

    PubMed  CAS  Google Scholar 

  18. Klatskin G., Kantor FS: Mitochondrial antibody in primary biliary cirrhosis and other diseases. Ann Intern Med 77: 533, 1972

    PubMed  CAS  Google Scholar 

  19. Whittingham S.: Immunofluorescence reactions in patients with prolonged extrahepatic biliary obstruction. Gastroenterology 66: 169, 1974

    PubMed  CAS  Google Scholar 

  20. Doniach D., Walker G.: Mitochondrial antibodies (AMA). Gut 15: 664, 1974

    Article  PubMed  CAS  Google Scholar 

  21. Winter SL, Kraft SC, Boyer JL: Antimitochondrial antibodies: reagent variables may lead to diagnostic error. Digestive Diseases and Sciences 24: 15, 1979

    Article  PubMed  CAS  Google Scholar 

  22. Boyden SV: The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med 93: 107, 1951

    Article  PubMed  CAS  Google Scholar 

  23. Witebsky E., Rose NR: Studies on organ specificity. IV. Production of rabbit thyroid antibodies in the rabbit. J Immunol 76: 408, 1956

    PubMed  CAS  Google Scholar 

  24. Roitt IM, Doniach D.: Human auto-immune thyroiditis: Serological studies. Lancet 2: 1027, 1958

    Article  PubMed  CAS  Google Scholar 

  25. Berg PA, Binder T: Demonstration of mitochondrial antibodies in sera from patients with chronic liver diseases reacting with two different types of complement-fixing antigens, in “Chronic hepatitis” Int Symp Montecatini 1975, by Gentilini P, Popper H, Tendori U. Basal: Karger, 1976, p 79

Download references

Author information

Authors and Affiliations

  1. The Third Division, Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1, 951, Niigata, Japan

    Sunao Katono M.D.

Authors
  1. Sunao Katono M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Katono, S. A comparative study of passive hemagglutina tion and immunofluorescence methods for the detection of mitochondrial antibodies in sera from patients with liver diseases. Gastroenterol Jpn 15, 233–240 (1980). https://doi.org/10.1007/BF02774273

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02774273

Key words

Search

Navigation