Skip to main content

An efficient protocol for genomic DNA extraction fromCitrus species

Abstract

We describe a simple and efficient method for genomic DNA extraction from woody fruit crops containing high polysaccharide levels. This method involves a modified CTAB or SDS procedure employing a purification step to remove polysaccharides by using water-saturated ether and 1.25 M NaCl. Precipitation with an equal volume of isopropanol caused a DNA pellet to form. After being washed with 70% ethyl alcohol, the pellet easily dissolved in TE buffer. Using this method, DNA was extracted from samples of more than 1000Citrus spp., including young leaves, old leaves, frosted old leaves, withered old leaves, and callus lines. The average yield of DNA ranged from 50–500 μg/g of sample. DNA was suitable for PCR and RFLP analyses and long-term storage. Recently, the procedure was used to isolate DNA from withered old leaves of more than 20 tropical and subtropical fruit crops.

This is a preview of subscription content, access via your institution.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Xiuxin Deng.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Cheng, YJ., Guo, WW., Yi, HL. et al. An efficient protocol for genomic DNA extraction fromCitrus species. Plant Mol Biol Rep 21, 177–178 (2003). https://doi.org/10.1007/BF02774246

Download citation

Key words

  • DNA extraction
  • fruit crops
  • polysaccharides
  • RFLP
  • water-saturated ether