Skip to main content

Advertisement

SpringerLink
Log in

The influence of cutting the pancreatic nerve plexus on exocrine function in the pancreas of rats

  • Original Article
  • Published:
Gastroenterologia Japonica Aims and scope Submit manuscript

Summary

This study was performed to clarify changes in protein synthesis and exocrine enzymes of the pancreas after cutting the pancreatic nerve plexus of Wistar rats. The rats were divided into two groups, consisting of a group that underwent cutting of the pancreatic nerve plexus (neurotomy group) and a group that underwent a sham operation (control group).3H-leucine uptake in the pancreatic protein fraction of the neurotomy group at 3, 5 and 7 days after the operation was significantly lower than that of the control group (P<0.05-0.01), and this low uptake returned to the normal range at 14 days. Amylase, lipase and trypsin values in pancreatic tissue of the neurotomy group decreased during the period of from 1 to 7 days, and there were significant differences in the values of the respective enzymes at 5 or 7 days between the neurotomy and the control group (P<0.05). Thereafter, all enzyme values increased to within the normal range. Upon examination of pancreatic blood flow using a microsphere, the neurotomy group showed a significant reduction at 7 days compared with the control group (P<0.05), and thereafter exhibited recovery of blood flow. These results indicate that after cutting the pancreatic nerve plexus, exocrine function in the pancreas is reduced immediately but recovers within a short period of time, and that these changes in exocrine function are affected by blood flow in the pancreas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Yoshioka H, Wakabayashi T: Traitement de la douleur des pancreatites chroniques par la neurotomie de la tete du pancreas. Une technique nouvelle et ses resultats. Lyon Chir 1957;53:836–845

    PubMed  CAS  Google Scholar 

  2. Mallet-Guy PA: Late and very late results of resection of the nervous system in the treatment of chronic relapsing pancreatitis. Am J Surg 1983;145:234–238

    Article  PubMed  CAS  Google Scholar 

  3. Hiraoka T, Tashiro S, Watanabe E, et al: Surgical treatment of chronic pancreatitis without pancreatic duct dilation. In: Sato T, Yamauchi H, eds. Pancreatitis Its Pathophysiology and Clinical Aspects. University of Tokyo Press, Tokyo. 1985;417–424

    Google Scholar 

  4. Hoffmann J, Jensen HE: Selective denervation of the pancreas for the pain of chronic pancreatitis. J Royal College Surgeous Edinburgh 1986;31:37–39

    CAS  Google Scholar 

  5. Pincus IJ, Thomas JE, Lachman PO: The effect of vagotomy on the secretion of pancreatic juice after the ingestion of various foodstuffs. Fed Proc 1948,1:94

    Google Scholar 

  6. Thistlethwaite JR: The effect of banthine, vagotomy, and subtotal gastrectomy upon pancreatic secretion. Surg Gynecol Obstet 1951;93:616–620

    PubMed  CAS  Google Scholar 

  7. Magee DF, Hayama T, White TT: The role of automonic nerves in the external secretion of the pancreas. Fed Proc 1962;321:265

    Google Scholar 

  8. Crider JO, Thomas JE: Secretion of pancreatic juice after cutting the extrinsic nerves. Am J Physiol 1944;141:730–737

    Google Scholar 

  9. Magee DF, Naruse S: Neural control of periodic secretion of the pancreas and the stomach in fasting dogs. J Physiol 1983;344:153–160

    PubMed  CAS  Google Scholar 

  10. Singer MV, Niebel W, Kniesburges S, et al: Action of atropine on the pancreatic secretory response to secretin before and after cutting the extrinsic nerves of the pancreas in dogs. Gastroenterology 1986,90:355–361

    PubMed  CAS  Google Scholar 

  11. Karnovsky MJ, Roots L: A direct-coloring thiocholine method for cholinesterases. J Histochem Cytochem 1964;12:219–221

    PubMed  CAS  Google Scholar 

  12. Nakamura T: Application of the Falgu method (Furness et al.) for the histochemical demonstration of catecholamine to the cryostat section method. Acta Histochem Cytochem 1979;12:182

    Google Scholar 

  13. Saini RK, Somani P:Hemodynamic and regional blood flow studies in the normotensive and spontaneously hypertensive rat: application of the radioactive microsphere technique. J Pharmachol Methods 1979;2:357–369

    Article  Google Scholar 

  14. Lowry OH, Rosebrough NJ, Farr AM, et al: Protein measurement with folin phenol reagent. J Biol Chem 1951;193:265–276

    PubMed  CAS  Google Scholar 

  15. Tiscornia OM: The neural control of exocrine and endocrine pancreas. Am J Gastroenterol 1977;67:541–560

    PubMed  CAS  Google Scholar 

  16. Tiscornia OM, Dreiling DA, Yacomotti J, et al: Neural control of the exocrine pancreas: an analysis of the cholinergic, adrenergic, and peptidergic pathways and their positive and negative components 1: neural mechanisms. Mount Sinai J Med 1987;54:366–383

    CAS  Google Scholar 

  17. Harper AA, Vass CCN: The control of the external secretion of the pancreas in cats. J Physiol 1941;99:415–435

    PubMed  CAS  Google Scholar 

  18. Goodhead B, Wright PW: The effect of postganglionic sympathectomy on the development of hemorrhagic pancreatitis in the dog. Ann Surg 1969;170:951–960

    Article  PubMed  CAS  Google Scholar 

  19. Barlow TE, Greenwell JR, Harper AA, et al: The effect of adrenaline and noradrenaline on the blood flow, electrical conductance and external secretion of the pancreas. J Physio 1971;217:665–678

    CAS  Google Scholar 

  20. Jansson L, Hellerstrom C: Intrinsic regulation of the blood flow to the endocrine and exocrine parts of the rat pancreas. Upsala J Med Sci 1987;92:233–241

    Article  PubMed  CAS  Google Scholar 

  21. Donahue PE, Ferguson JL, Filho PG, et al: Effect of chemical sympathectomy with oxidopamine on systemic blood flow in rats with acute pancreatitis. Circulatory Shock 1986;19:23–30

    PubMed  CAS  Google Scholar 

  22. Richins CA: Effect of sympathetic nerve stimulation on blood flow and secretion in the pancreas of the cat. Am J Physiol 1953;173:467–470

    PubMed  CAS  Google Scholar 

  23. Tankel HL, Hollander F: The relation between pancreatic secretion and local blood flow. Gastroenterology 1957,32:633–641

    PubMed  CAS  Google Scholar 

  24. Eicheiter P, Schenk WG: Hemodynamics of pancreatic secretion. Arch Surg 1966;93:200–207

    Google Scholar 

  25. Papp M, Varga B, Folly G: Effect of secretin, pancreozymin, histamine, decholin on canine pancreatic blood flow. Pflügers Arch 1973;340:349–360

    Article  PubMed  CAS  Google Scholar 

  26. Tsuruoka M: The effect of the pancreatic blood flow on exocrine secretion of the canine pancreas. Japanese J Gastroenterology 1976;73:1056–1069

    CAS  Google Scholar 

  27. Hickson JCD: The secretory and vascular response to nervous and hormonal stimulation in the pancreas of the pig. J Physiol (Lond.) 1970;206:299–322

    CAS  Google Scholar 

  28. You CH, Rominger JM, Chey WY: Effect of atropine on the action and release of secretin in humans. Am J Physiol 1982;242:G608-G611

    PubMed  CAS  Google Scholar 

  29. Korc M: Regulation of pancreatic protein synthesis by cholecystokinin and calcium. Am J Physiol 1982;243:G69-G75

    PubMed  CAS  Google Scholar 

  30. Dreiling DA, Druckerman LJ, Hollander F: The effect of complete vagisection and vagal stimulation on pancreatic secretion in man. Gastroenterology 1952;20:578–586

    PubMed  CAS  Google Scholar 

  31. Routley EF, Mann FC, Bollman JL, et al: Effect of vagotomy on pancreatic secretion in dogs with chronic pancreatic fistula. Surg Gynecol Obstet 1952;95:529–536

    CAS  Google Scholar 

  32. Wang CC, Grossman MI: Physiological determination of release of secretin and pancreozymin from intestine of dogs with transplanted pancreas. Am J Physiol 1951;164:527–545

    PubMed  CAS  Google Scholar 

  33. Augier D, Boucard JP, Pascal JP, et al: Relationships between blood flow and secretion in the isolated perfused canine pancreas. J Physiol 1972;221:55–69

    PubMed  CAS  Google Scholar 

  34. Radke R, Stach W: Innervation of the canine pancreas after vagotomy. Acta Anat 1986;127:88–92

    Article  PubMed  CAS  Google Scholar 

  35. Warren KW: Pathologic considerations as a guide to the choice of surgical procedures in the management of chronic relapsing pancreatitis. Gastroenterology 1959;36:224–231

    PubMed  CAS  Google Scholar 

  36. Tsuchiya R, Yamaguchi T, Tsunoda T: Indication of surgical procedures for chronic pancreatitis. J Clinical Surg 1981;36: 1599–1606

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Second Department of Surgery, Kanazawa Medical University, Uchinada-Machi, Kahoku-Gun, 920-02, Ishikawa, Japan

    Yoshio Kinami M.D. & Hiroshi Sakuma

Authors
  1. Yoshio Kinami M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Sakuma
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported partially by the grants from the Japanese Ministry of Health & Welfare.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kinami, Y., Sakuma, H. The influence of cutting the pancreatic nerve plexus on exocrine function in the pancreas of rats. Gastroenterol Jpn 24, 549–555 (1989). https://doi.org/10.1007/BF02773884

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02773884

Key words

Search

Navigation