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Campylobacter pylori in Japan: Bacteriological feature and prevalence in healthy subjects and patients with gastroduodenal disorders

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Summary

The presence ofCampylobacter pylori was investigated in biopsy specimens obtained during gastrofiberscopy from 103 consecutive patients prospectively. Patients included 25 with gastric ulcer, 4 with duodenal ulcer, 5 with coexisting gastroduodenal ulcer, 31 with gastroduodenal ulcer with gastritis, 27 with gastritis, 3 with gastric polyps and 8 with gastric cancer. Results were compared with 20 healthy control subjects who were endoscopically normal. Two specimens each were taken from 3 sites in the stomach. One part was used for a histological study to examine the presence of the organisms. The other part was cultured using Skirrow’s agar microaerophilically. Conventional microflora andC. pylori were examined in gastric contents of some cases. Bacteriological features of isolated strains of C.pylori were identical to the NCTC strain. C.pylori was the most dominant organism in gastric contents at any pH level. Detection rates of C.pylori by bacteriological culture were 96% in gastric ulcer, 100% in duodenal ulcer, 80% in coexisting gastroduodenal ulcer, 84% in gastroduodenal ulcer with gastritis, 70% in gastritis, 100% in gastric polyps and 100% in gastric cancer, and the percentages recognized by histological studies were 81,100,100, 84, 71, 67, and 57%, respectively. The values in healthy controls were 55% by histological and bacteriological methods (P<0.001 compared with overall ulcer patients). These results supported the close association between C.pylori and gastroduodenal diseases.

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References

  1. Warren JR, Marshall BJ: Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983;i:1273–1275

    Google Scholar 

  2. Warren JR, Marshall BJ: Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984;i:1311–1314

    Google Scholar 

  3. Jones DM, Lessells AM, Eldridge J: Campylobacter like organisms on the gastric mucosa; culture, histological, and serological studies. J Clin Pathol 1984;37:1002–1006

    Article  PubMed  CAS  Google Scholar 

  4. Phillips AD, Hine KR, Holmes GKT, et al: Gastric spiral bacteria. Lancet 1984;ii:100–101

    Google Scholar 

  5. Thomas MJ, Poynter D, Gooding C, et al: Gastric spiral bacteria. Lancet 1984;ii:100

    Google Scholar 

  6. Langenberg ML, Tytgat GNJ, Schipper MEI, et al: Campylobacter-like organisms in the stomach of patients and healthy individuals. Lancet 1984;i:1348

    Article  Google Scholar 

  7. Lopez-Brea M, Jimenez ML, Blanco M, et al: Isolation of Campylobacter pyloridis from patients with and without gastroduodenal pathology. In: Pearson AD, Skirrow MB, Lior H, eds. Campylobacter III. Proceedings of the Third International Workshop on Campylobacter Infections, Public Health Laboratory Service, London. 1984;193–194

    Google Scholar 

  8. Pettross CW, Cohen H, Appleman MD, et al: Campylobacter pyloridis: relationship to peptic disease, gastric inflammation and other conditions. Gastroenterology 1986;90:1585

    Google Scholar 

  9. Drumm B, O’Brien A, Cutz E, et al: Campylobacter pyloridis are associated with primary antral gastritis in the pediatric population. Gastroenterology 1986;90:1399

    Google Scholar 

  10. Marcheggiano A, Iannoni C, Agnello M, et al: Campylobacterlike organisms gastritis and peptic ulcer. Gastroenterology 1986;90:1533

    Google Scholar 

  11. Inoue H, Ishii E, Tsuyuguchi T, et al: Rod bacteria like shaped particles in the biopsy specimens of human stomach mucosa, searching for Campylobacter like organisms (CLO). JJA Inf D 1985;59:163 (suppl) (in Jpn)

    Google Scholar 

  12. Ishii E, Kizumi T, Inoue H, et al: Isolation of Campylobacter pyloridis from gastric mucosa of patients with gastric complaints and susceptibility of the isolates on antibiotics. JJA Inf D 1987;61:668–675 (in Jpn)

    CAS  Google Scholar 

  13. Amano K: Relationship between microflora of the upper small intestine and steatorrhoea in patients with extensive gastric resection. Jpn J Gastroenterol 1987;84:1591–1604 (in Jpn)

    CAS  Google Scholar 

  14. Laemmli, UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–685

    Article  PubMed  CAS  Google Scholar 

  15. Inoue H, Ishii E, Tanaka T, et al: Clinical and bacteriological aspects of Campylobacter-like-organism (CLO) or Campylobacter pyloridis in the case of gastric illness in Japan. Dig Dis Sci 1986;31:149s

    Google Scholar 

  16. Matsui K, Tamura K, Terada A, et al: Campylobacter pylori in gastric diseases. Jpn J Gastroenterol 1987;84:1864 (in Jpn)

    CAS  Google Scholar 

  17. Kochiyama T: Clinical study of Campylobacter pylori in stomach disease. Gastroenterol Endosc 1989;31:3–13 (in Jpn)

    Google Scholar 

  18. Pearson AD, Ireland A, Bamforth J, et al: Polyacrylamide gel electrophoresis of spiral bacteria from the gastric antrum. Lancet 1984;i:1349–1350

    Article  Google Scholar 

  19. Ito T, Yanagawa Y, Shingaki M, et al: Isolation of Campylobacter pyloridis from human gastric mucosa and characterization of the isolates. Microbiol Immunol 1987;31:603–614

    Google Scholar 

  20. Marshall BJ, Royce H, Annear DI, et al: Original isolation of Campylobacter pyloridis from human gastric mucosa. Microbios 1984;25:83–88

    Google Scholar 

  21. Hazell SL, Lee A: Campylobacter pyloridis, urease, hydrogen ion back diffusion, and gastric ulcers. Lancet 1986;ii:15–17

    Article  Google Scholar 

  22. Kent TH, Cardelli RM, Stamler FW: Small intestinal ulcers and intestinal flora in rats given indomethacin. Am J Pathol 1969;45:237–245

    Google Scholar 

  23. Fang W, Broughton A, Jacobsen ED: Indomethacin-induced intestinal inflammation. Am J Dig Dis 1977;22:749–760

    Article  PubMed  CAS  Google Scholar 

  24. Robert A, Asano T: Resistance of germfree rats to indomethacin-induced intestinal lesions. Prostaglandins 1977;14: 333–341

    Article  PubMed  CAS  Google Scholar 

  25. Satoh H, Guth PH, Grossman MI: Role of bacteria in gastric ulceration produced by indomethacin in the rat: cytoprotective action of antibiotics. Gastroenterology 1983;84:483–489

    PubMed  CAS  Google Scholar 

  26. Giannela RA, Broitman SA, Zamcheck N: Gastric acid barrier to ingested micro organism in man: studies in vivo and in vitro. Gut 1972;13:251–256

    Article  Google Scholar 

  27. Hazell SL, Lee A Brady L, et al: The adaptation of motile strains of Campylobacter pyloridis to gastric mucus and their association with gastric epithelial intercellular space. J Infect Dis 1986;153:658–663

    PubMed  CAS  Google Scholar 

  28. Allen A, Garner G: Mucus and bicarbonate secretion in the stomach and their possible role in mucosa] protection. Gut 1980;21:249–262

    Article  PubMed  CAS  Google Scholar 

  29. Marshall BJ, McGechie DB, Francis GJ: Pyloric campylobacter serology. Lancet 1984;2:281

    Article  PubMed  CAS  Google Scholar 

  30. Bizzozero G: Ueber die schlauchformigen Drusen des Magendarmkanals und die Bezienhungen ihres Epithels zu dem Oberflachenepithel der Schleimhaut. Arch f Mikr Anat 1893;42:82–152

    Article  Google Scholar 

  31. Doenges JL: Spirochaetes in gastric glands of macacus rhesus and humans without definite history of related disease. Proc Soc Exp Med Biol 1938;38:536–538

    Google Scholar 

  32. Baskerville A, Newell DG: Naturally occurring chronic gastritis and C. Pylorinfection in the Rhesus monkey: a potential model for gastritis in man. Gut 1988;29:465–472

    Article  PubMed  CAS  Google Scholar 

  33. Fox JG, Edrise BM, Cabot EB: Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am J Vet Res 1986;47:236–239

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The Department of Internal Medicine 4, Hyogo College of Medicine, 1-1 Mukogawacho, 663, Nishinomiya, Hyogo, Japan

    Hiroyuki Inouye M.D., Issei Yamamoto, Noritoshi Tanida, Jun Mikami, Kazutami Tamura, Tadatsugu Ohno, Masakatsu Kano & Takashi Shimoyama

Authors
  1. Hiroyuki Inouye M.D.
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  2. Issei Yamamoto
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  3. Noritoshi Tanida
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  4. Jun Mikami
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  5. Kazutami Tamura
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  6. Tadatsugu Ohno
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  7. Masakatsu Kano
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  8. Takashi Shimoyama
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Inouye, H., Yamamoto, I., Tanida, N. et al. Campylobacter pylori in Japan: Bacteriological feature and prevalence in healthy subjects and patients with gastroduodenal disorders. Gastroenterol Jpn 24, 494–504 (1989). https://doi.org/10.1007/BF02773875

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  • DOI: https://doi.org/10.1007/BF02773875

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