Summary
Two types of experimental chronic pancreatic damage were induced in rats by a copper-deficient diet containing D-penicillamine (DP rats) and by a similar diet without D-penicillamine (D rats). At 5–6 weeks after commencement of treatment, serum copper levels showed a significant decrease in both DP rats (1.5 ± 0.9 Μg/dl, M ± SE) and D rats (18.8 ± 1.4 Μg/dl) compared with the findings in control rats (111.8 ± 4.4 Μg/dl). Histology revealed loss and atrophy of acinar cells in DP rats and mild vacuolar degeneration of acinar cells in D rats, whereas the islets of Langerhans were intact in both groups. The secretion of the exocrine pancreas showed a marked decrease in enzymes and protein output in DP and D rats compared with findings in the control rats. Our study confirmed the relationships between serum copper levels and morphological damage of pancreatic acinar cells or the reduction of enzyme secretion. On the other hand, the reductions in volume and bicarbonate output of pancreatic juice in DP rats and in D rats were detected, but no relationship was found between these reductions and the serum copper levels. The endocrine pancreatic function in DP rats showed a slight disturbance in intravenous glucose tolerance test. These results support the conclusion that copper-deficiency causes disturbances in enzyme-synthesis and selective destruction of acinar cells.
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References
Wakasugi H, Nakayama K, Kimura T, et al: Effect of long-term administration of ethanol on the exocrine pancreatic secretion in rats. Medicine and Biology 1977, 94: 471–474 Jpn)
Almeida AL, Grossman MI: Experimental production of pancreatitis with ethionine. Gastroenterology 1952, 20: 554–577
Kimura T, Imamura K, Matsumoto M, et al: Studies on serum immunoreactive elastase in experimental pancreatitis rats. II. In chronic pancreatitis. Gastroenterol Jpn 1980, 15: 625–629
Kubota K: Exocrine pancreatic secretion in the pancreatitis dogs induced by ductule obstruction with microspheres. The Biliary Tract & Pancreas 1980, 4: 1347–1357 (Jpn)
Hayashida Y, Gonda H, Yokota H, et al: Experimental pancreatitis. Igaku-no-Ayumi 1977, 103: 346–352 (Jpn)
Frieden E, Osaki S, Kobayashi H: Copper proteins and oxygen. Correlations between structure and function of the copper oxidases. J Gen Physiol 1965, 49: 213–252
Perings E, Brunner G, Bokkermann M, et al: Lipomatose des exokrinen Pankreas nach D-Penicillamine-Futterrung bei Ratten unter Cholinemangeldiat. Dtsch Med Wochenshr 1973, 98: 1085–1086
Müller HB: Der Einfluss kupferarmen Kost auf das Pankreas Lichtmikroskopische Untersuchungen am exokrinen Teil der Bauchspeicheldrusen weiseer Ratten. Virchows Arch Abt A path Anat 1970, 350: 353–367
Fölsch UR, Creutzfelt W: Pancreatic duct cells in rats; secretory studies in response to secretin, cholecystokinin-pancreozymin and gastrin in vivo. Gastroenterology 1977, 73: 1053–1059
Ceska M, Briath K, Brown BA: A new rapid method for clinical determination of alpha-amylase activities in human serum and urine. Optimal conditions. Clin Chim Acta 1969, 26: 437–444
Bieth J, Spiess B, Wermutha CG: The synthesis and analytic use of a highly sensitive and convenient substrate of elastase. Biochem Med 1974, 11: 350–357
Lowry OH, Rosebrough NJ, Farr AL, et al: Protein measurement with folin phenol reagent. J Biol Chem 1951, 193: 265–275
Fell BF, King TP, Davies NT: Pancreatic atrophy in copper-deficient rats; histochemical and ultrastructural evidence of a selective effect on acinar cells. Histochemical Journal 1982, 14: 665–680
Shwachman H, et al: The syndrome of pancreatic insufficiency and bone marrow dysfunction. J Pediatrics 1964, 65: 645–663
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Kimura, T., Sumii, T., Oogami, Y. et al. Morphological and biochemical changes in the pancreas of copper-deficient rats. Gastroenterol Jpn 22, 480–486 (1987). https://doi.org/10.1007/BF02773817
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DOI: https://doi.org/10.1007/BF02773817