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Human bile duct carcinoma cell line producing abundant mucinin vitro

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Summary

A human bile duct carcinoma cell line, designated OZ, was established from ascitic effusion of a patient who suffered from obstructive jaundice due to the clogging of the common bile duct with mucinous substances secreted by the cancer cells. OZ was found to be capable of producing mucin in vitro and pools of mucin were macroscopically identified on the monolayer of the cells. On the electron micrographs, cell coat type mucin and abundant intracytoplasmic desmosomes were observed. The OZ cells secreted cardnoembryonic antigen in culture and had high enzymatic activity of γ-glutamyl transpeptidase. The tumor heterotransplanted into nude mice also showed mucin production.

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References

  1. Braasch JW, et al: Malignant neoplasms of the bile ducts. Surg Clin North Am 1967, 47: 627

    PubMed  CAS  Google Scholar 

  2. Okuda K, et al: Clinical aspects of intrahepatic bile duct carcinoma including hilar carcinoma. Cancer 1977, 39: 232

    Article  PubMed  CAS  Google Scholar 

  3. Kage M, et al: Mucin producing cholangiocarcinoma with obstructive jaundice: Report of two cases. Acta Hepatol Jpn 1980, 21: 1068

    Google Scholar 

  4. Oota T, et al: A case of mucinous biliary obstruction associated with multiple cholangiocarcinoma. The Biliary Tract and Pancreas 1983, 4: 687

    Google Scholar 

  5. Moorhead PS, et al: Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). Expel Cell Res 1965, 39: 190

    Article  CAS  Google Scholar 

  6. Bessey OA, et al: Amethod for the rapid determination of alkaline-phosphatase with five cubic millimeters of serum. J Biol Chem 1946, 164: 321

    CAS  Google Scholar 

  7. Goldbarg LA, et al: The colorimetric determination of γ-glutamyl transpeptidase with the synthetic substrate. Arch Biochem Biophys 1960, 91: 61

    Article  PubMed  CAS  Google Scholar 

  8. Lowry OH, et al: Protein measurement with the folic phenol reagent. J Biol Chem 1951, 193: 265

    PubMed  CAS  Google Scholar 

  9. Homma S, et al: Histological similarity of nude mice transplanted tumor to the original human hepatobiliary carcinomas. Jikeikai Med J 1985, 32: 317

    Google Scholar 

  10. Okumura H, et al: Cell colony formation and cloning. In: National Institute of Health, eds. The methodology of virological experiments. Marzen Press, Tokyo, 1964, 149

    Google Scholar 

  11. Homma S, et al: Studies on the establishment and some biological characteristiocs of the cultured human liver cancer cell lines. Jikeilai Med J 1985, 32: 289

    Google Scholar 

  12. Fogh J, et al: New human tumor cell lines. In: Fogh J, ed. Human tumor cellsin vitro. Plenum Press, New York, 115

  13. Chen WH, et al:In vitro andin vivo morphology of a new tumor cell line established from ascites.In vitro 1982, 18: 24

    PubMed  CAS  Google Scholar 

  14. Sherlock S, et al: Tumors of the gallbladder and bile ducts. In: Sherlock S, ed. Diseases of the liver and biliary system 6th ed. Blackwell scientific publications, London, 1981, 510

    Google Scholar 

  15. Chou ST, et al: Mucin producing cholangiocarcinoma. Pathology 1976, 8: 321

    Article  PubMed  CAS  Google Scholar 

  16. Sanes S, et al: Primary carcinoma of the liver. Cholangioma in hepatolithiasis. Am J Pathol 1942, 18: 675

    Google Scholar 

  17. Johnson RR, et al: Cystadenocarcinoma of the pancreas with a staged pancreatoduodenectomy. NY State J Med 1975, 74: 865

    Google Scholar 

  18. God A, et al: A histochemical study of human alimentary tract mucosubstances in health and disease. I Normal and tumors. Br J Cancer 1974, 23

  19. Filipe MI, et al: Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut 1969, 10: 577

    Article  PubMed  CAS  Google Scholar 

  20. Korhonen LK, et al: Carbohydrate-rich compounds in the colonic mucosa of man. II Histochemical characteristics of colonic adenocarcinomas. Cancer 1971, 27: 128

    Article  PubMed  CAS  Google Scholar 

  21. Lev R, et al: Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem 1964, 12: 309

    PubMed  CAS  Google Scholar 

  22. Pease AGE: Carbohydrate and mucosubstances. In: Pease AGE, ed. Histochemistry. Theoretical and applied Vol I. J.A. Churchill, London, 1968, 294

    Google Scholar 

  23. Ito S: Form and function of the glycocalyx on free cell surface coat. Phil Trans R Soc Lond B 1974, 268: 55

    Article  Google Scholar 

  24. Luft JH: The structure and properties of the cell surface coat. Int Rev Cytol 1976, 45: 291

    Article  PubMed  CAS  Google Scholar 

  25. Macnab GM, et al: Hepatitis B surface antigen produced by a human hepatoma cell line. Br J Cancer 1976, 34: 509

    PubMed  CAS  Google Scholar 

  26. Knowles BB, et al: Human hepatocellular carcinoma cell lines secrete the major plasma protein and hepatitis B surface antigen. Science 1980, 209: 497

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. First Department of Internal Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minatoku, 105, Tokyo, Japan

    Sadamu Homma M.D., Scishi Nagamori M.D., Kiyotaka Fujise M.D., Kazunobu Yamazaki M.D., Satoshi Hasumura M.D., Hajime Sujino M.D., Tomokazu Matsuura M.D., Keiichiro Shimizu M.D. & Haruo Kameda M.D.

  2. Department of Pathology, The Jikei University School of Medicine, 105, Tokyo, Japan

    Keizo Takaki M.D.

Authors
  1. Sadamu Homma M.D.
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  2. Scishi Nagamori M.D.
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  3. Kiyotaka Fujise M.D.
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  4. Kazunobu Yamazaki M.D.
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  5. Satoshi Hasumura M.D.
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  6. Hajime Sujino M.D.
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  7. Tomokazu Matsuura M.D.
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  8. Keiichiro Shimizu M.D.
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  9. Haruo Kameda M.D.
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  10. Keizo Takaki M.D.
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Additional information

This study was supported in part by a Grant-in-Aid for cancer research (58-19) form the Ministry of Health and Welfare.

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Homma, S., Nagamori, S., Fujise, K. et al. Human bile duct carcinoma cell line producing abundant mucinin vitro . Gastroenterol Jpn 22, 474–479 (1987). https://doi.org/10.1007/BF02773816

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  • DOI: https://doi.org/10.1007/BF02773816

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