Advertisement

Gastroenterologia Japonica

, Volume 15, Issue 1, pp 41–48 | Cite as

The effects of intravenously administered chlorophyll-a on naturally occurring serum protease inhibitors in rabbits

  • Akira Yoshida
  • Osamu Yokono
  • Toshitsugu Oda
Original Article
  • 20 Downloads

Summary

Effects of intravenously administered protease inhibitors on naturally occurring serum inhibitors were investigated in rabbits. Water-dispersed chlorophyll-a, trasylol and leupeptin were tested as exogenous protease inhibitors in the experiments. From the results of experiments, it was concluded that:
  1. 1)

    Pretreatment with chlorophyll-a infusion into rabbits, most effectively prevented the rapid consumption of naturally occurring serum protease inhibitors after successive trypsin infusion, and the duration of its action was observed longest when compared with other exogenous inhibitors, such as trasylol or leupeptin.

     
  2. 2)

    Final therapeutic effects of these exogenous protease inhibitors seem to have depended upon the disappearing way of the administered inhibitors from the blood in certain period of time, as well as upon biochemical potency of their inhibiting activity.

     

Key Words

pancreatitis chlorophyll-a protease inhibitors 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Vogel R, Trautschold I, Werle E: Natürliche Proteinasen-Inhibitoren pp 39–50 Georg Thieme Verlag, Stuttgart, 1966Google Scholar
  2. 2).
    Barnhart MI, Quintana C, Lenon HL, Bluhm GB, Riddle JM: Proteases in inflammation. Ann NY Acad Sci 146: 527, 1968PubMedCrossRefGoogle Scholar
  3. 3).
    Wuepper KD: Biochemistry and biology of components of the plasma kinin-forming system, In: Inflammation pp 93, Lepow, I.H. and Ward, P.A. ed. Acad. Press, New York, 1972Google Scholar
  4. 4).
    Creutzfeldt W, Schmidt H: Aetiology and Pathogenesis of pancreatitis (Current concepts). Scand J Gastroenterol 5 (Suppl. 6): 47–62, 1970Google Scholar
  5. 5).
    Vogel R, Trautschold I, Werle E: Der Polyvalente Inhibitor der Rinder Organe, In: Naturliche Proteinasen-Inhibitoren pp 52, Georg Thieme Verlag, Stuttgart, 1966Google Scholar
  6. 6).
    Oda T, Yokono O, Yoshida A, et al: The effect of chlorophyll-a in the treatment of pancreatitis. Nihon Iji Shimpo 2438, 1969 (in Jpn)Google Scholar
  7. 7).
    Oda T, Yokono O, Yoshida A, Miyake K, lino S: On the successful treatment of pancreatitis with chlorophyll-a and inhibitory effects of its derivatives on trypsin and other protease activities in vitro. Gastroenterol Jpn 6: 49, 1971Google Scholar
  8. 8).
    Aoyagi T, Miyata S, Umezawa H, et al: Biological activities of leupeptins. J Antibiotics 22: 558, 1969Google Scholar
  9. 9).
    Anson ML, Mirsky, AE: The estimation of trypsin with hemoglobin. J Gen Physiol 117: 151, 1934Google Scholar
  10. 10).
    Vogel R, Trautschold I, Werle E: Inhibitor aus menschlichem Harn, In: Naturliche Proteinasen-Inhibitor pp 50, Georg Thieme Verlag, Stuttgart, 1966Google Scholar
  11. 11).
    Innerfield I, Angrist A, Benjamin J: Plasma antithrombin patterns in pancreatic disturbances. Gastroenterology 20: 417, 1951Google Scholar
  12. 12).
    Corrado AP, Reis IF, Calvalho IF, Diniz CR: Bradykininogen and bradykinin in the cardiovascular shock produced by proteolytic enzymes. Biochem Pharmacol 15: 959, 1966PubMedCrossRefGoogle Scholar
  13. 13).
    Yoshida A, Yokono O, Oda T: Therapeutic effect of chlorophyll-a in the treatment of patients with chronic pancreatitis, submitted for publication to Gastroenterol Jpn, 1979Google Scholar
  14. 14).
    Peacock AC, Sheehy JJ: Studies of various tests for malignant neoplastic diseases VII. Serum inhibitors of chymotrypsin and trypsin. J Natl Cancer Inst 12: 861, 1952PubMedGoogle Scholar
  15. 15).
    Kueppers F: Genetically determined differences in the responce of alphai-antitrypsin levels in human serum to typhoid vaccine. Humangenetik 6: 207, 1968PubMedCrossRefGoogle Scholar
  16. 16).
    Lieberman J, Mittman C: Dynamic response of alphai-antitrypsin variants to diethylstilbestrol. Am J hum Genet 25: 610, 1973PubMedGoogle Scholar
  17. 17).
    Werle E, Trautschold I, Haendle H, Fritz H: Physiologic, pharmacologic, and clinical aspects of proteinase inhibitor. Ann NY Acad Sci 146: 464, 1968PubMedCrossRefGoogle Scholar
  18. 18).
    Taoka Y: Studies on absorption, excretion and distribution in the body of exogenous chlorophyll-a. Igakunoayumi (Advances in Medicine) 91: 241, 1974Google Scholar
  19. 19).
    Konno K, Takeda M: Experimental report of absorption, excretion and metabolism of chlorophyll-a. The Clinical Report 8: 2397, 1974Google Scholar
  20. 20).
    Miyagawa F, Noguchi T: Effect of pantethine and chlorophyll-a to the fatty liver induced with the carbon tetrachloride. The St. Marianna Medical Journal 6: 379, 1978.Google Scholar

Copyright information

© The Japanese Society of Gastroenterology 1980

Authors and Affiliations

  • Akira Yoshida
    • 1
  • Osamu Yokono
    • 2
  • Toshitsugu Oda
    • 1
  1. 1.The First Department of Medicine, Faculty of MedicineUniversity of TokyoTokyoJapan
  2. 2.Health Administration CenterTokyo University of Foreign StudiesJapan

Personalised recommendations