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Gastroenterologia Japonica

, Volume 15, Issue 1, pp 20–26 | Cite as

Studies on circulating immune complexes of the liver disease. 3. Conditions for Clq binding assay and low molecular weight Clq reactants

  • Terukatsu Arima
  • Jin Narumoto
  • Hiroyuki Shimomura
  • Kinichiro Suwaki
  • Kensuke Kunishi
  • Takashi Yasuhara
  • Hideo Nagashima
Original Article

Summary

Assay conditions for Clq binding activity (ClqBA) of aggregated human gamma globulin and pathologic sera were studied. The Clq component of complement was isolated by precipitation method and effectively radioiodinated using lactoperoxidase without loss of binding activity. The assay method was reproducible and the binding activity of normal sera was 5.0+-2.4% (mean +-S.D.). Amount of125I-Clq, in the range of 50–1000 ng per assay tube, did not influence the binding activity. Competitive inhibition of intrinsic Clq was very limited. Sample sera for the determination can be stored at -20‡C and freezing-thowing of the sera did not increase ClqBA. In normal sera, the existence of solubilizer of Clq-reactant-complex or inhibitor of Clqreactant binding working at 37‡C is suggested. For the low molecular weight reactants in pathologic sera reacting with Clq at 4‡C, following substances are proposed: 1. abnormal IgG, 2. hapten-IgG complexes or 3. anionic substances.

Key Words

Clq immune complexes aggregated human gamma globulin 7S-Clq reactants radioassay lactoperoxidase liver disease gel filtration 

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Copyright information

© The Japanese Society of Gastroenterology 1980

Authors and Affiliations

  • Terukatsu Arima
    • 1
  • Jin Narumoto
    • 1
  • Hiroyuki Shimomura
    • 1
  • Kinichiro Suwaki
    • 1
  • Kensuke Kunishi
    • 1
  • Takashi Yasuhara
    • 1
  • Hideo Nagashima
    • 1
  1. 1.The First Department of Internal MedicineOkayama University Medical SchoolOkayamaJapan

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