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A quick method to isolate RNA from wheat and other carbohydrate-rich seeds

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No reports on isolating RNA from carbohydrate-rich wheat seeds have been published. Because of the presence of carbohydrates, published protocols yield small amounts of poor quality RNA. Extracting seeds in a buffer (pH 9, 150 mM NaCl, 1% sarcosyl) ensured maximum RNA solubility and the removal of most interfering substances. Extracted RNA was purified using a guanidine hydrochloride-based buffer system. This protocol yields up to 148 μg of RNA from 100 mg of tissue in 3.5 h. An A260/A280 ratio of 1.85 indicates RNA purity. Isolated RNA was amenable to downstream applications such as differential display. The developed method was extended to other carbohydrate-rich seeds, such as barley and maize, with success.

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Singh, G., Kumar, S. & Singh, P. A quick method to isolate RNA from wheat and other carbohydrate-rich seeds. Plant Mol Biol Rep 21, 93 (2003).

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