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Improving transformation efficiency ofArabidopsis thaliana by modifying the floral dip method

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Abstract

Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of theAgrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2–3%.

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Abbreviations

GUS:

β-glucuronidase

MS:

Murashige and Skoog

RT-PCR:

reverse transcriptase-polymerase chain reaction

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Authors and Affiliations

  1. Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58066, Morelia, Michoacán, México

    Miguel Martinez-Trujillo

  2. Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Km 9.6 carretera Irapuato-León, 36500, Irapuato, Guanajuato, México

    Veronica Limones-Briones, José Luis Cabrera-Ponce & Luis Herrera-Estrella

Authors
  1. Miguel Martinez-Trujillo
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  2. Veronica Limones-Briones
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  3. José Luis Cabrera-Ponce
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  4. Luis Herrera-Estrella
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Martinez-Trujillo, M., Limones-Briones, V., Cabrera-Ponce, J.L. et al. Improving transformation efficiency ofArabidopsis thaliana by modifying the floral dip method. Plant Mol Biol Rep 22, 63–70 (2004). https://doi.org/10.1007/BF02773350

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