Abstract
Two DNA-based marker systems, amplified fragment legnth polymorphism (AFLP) and inverse sequence-tagged repeat (ISTR), were evaluated with respect to their discriminating capacity and efficiency in genetically analyzing henequen (Agave fourcroydes). Samples were taken from a mother plant, sucker-derived daughter plants, and bulbils. ISTR analysis produced more polymorphic markers than AFLP and had a better capacity for quantifying genetic diversity through an average number of alleles per locus, expected heterozygosis of polymorphic loci, expected heterozygosity, effective number of alleles per locus, and total number of effective alleles. ISTR also showed superior discriminatory capacity.
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Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- ISTR:
-
inverse sequence-tagged repeat
- PIC:
-
polymorphic information content
- RAPD:
-
random amplification of polymorphic DNA
- TBE:
-
buffer of Tris-base, boric acid, and EDTA
- SE:
-
somatic embryogenesis
References
Anderson JA, Churchill GA, Autrique JE, Tanksley SD, and Sorrells ME (1993) Optimizing parental selection for genetic linkage maps. Genome 36: 181–6.
Balzarini M, Di Rienzo J, and Casanoves F (2003) Info-Gen: Un ambiente integrado para el análisis de datos genéticos. In: II Meeting Caribean and Central American Region. International Biometrics Society, Puerto Rico, USA, pp 20
Buntjer J (2000) CrossChecker Fingerprint Analysis Software. Wageningen University and Research Center, The Netherlands.
Castorena-Sánchez I, Escobedo RM, and Quiroz A (1991) New cytotaxonomical determinants recognized in six taxa of Agave in the sections Rigidae and Sisalanae. Can J Bot 69: 1257–64.
Demey JR, Zambrano AY, Fuenmayor F, and Segovia V (2003) Relación entre las características moleculares y morfológicas en una colección de Yuca. Interciencia 28(12):684–9.
Gil-Vegas K, González-Chavira M, Martínez de la Vega O, Simpson J, and Vandermark G (2001) Analysis of genetic diversity inAgave tequilana var.Azul using RAPD markers. Euphytica 119: 335–41.
González G, Alemán S, and Infante D (2003) Asexual genetic variability inAgave fourcroydes II: selection among individuals in a clonally propagated population. Plant Sci 165: 595–601.
Infante D, González G, Peraza-Echeverría L, and Keb-Llanes M (2003) Asexual genetic variability inAgave fourcroydes. Plant Sci 164: 223–30.
Kahl G (1995) Dictionary of Gene Technology, pp 548. VCH Publishers, Inc., New York.
Keb-Llanes M, González G, Chi-Manzanero B, and Infante D (2002) A rapid and simple method for small scale DNA extraction in Agavaceae and other tropical plants. Plant Mol Biol Rep 20: 299a-e.
Martínez-Palacios A, Eguiarte LE, and Furnier GR (1999) Genetic diversity of the endangered endemic Agave victoriae-reginae (Agavaceae) in the Chihuahuan Desert. Am J Bot 86: 1093–8.
Morgante M, Rafalsky A, Biddle P, Tingey S, and Olivieri AM (1994) Genetic mapping and variability of seven soybean simple sequence repeat loci. Genome 37: 763–9.
Pejic I, Ajmone-Marsan P, Morgante M, Kozumplik M, Castiglioni P, Taramino G, and Motto M (1998) Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theor Appl Genet 97: 1248–55.
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, and Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2: 225–38.
Ramakrishna W, Lagu WD, Gupta VS, and Ranjekar PK (1994) DNA fingerprinting in rice using oligonucleotide probes specific for simple repetitive DNA sequences. Theor Appl Genet 88: 402–6.
Rohde W (1996) Inverse sequence-tagged repeat (ISTR) analysis, a novel and universal PCR-based technique for genome analysis in the plant and animal kingdom. J Genet and Breed 50: 249–61.
Sambrook J, Fritsch EF, and Maniatis T (1980) Molecular Cloning: A Laboratory Manual, pp I-I.47. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Sanchez-Teyer LF, Quiroz-Figueroa F, Loyola-Vargas V, and Infante D (2003) Culture-induced variation in plants of Coffea arabica cv. caturra rojo, regenerated by direct and indirect somatic embryogenesis. Mol Biotechnol 23: 107–15.
Sensi E, Vignani R, Rohde W, and Biricolti S (1996) Characterization of genetic biodiversity withVitis vinifera L. «Sangiovese» and «Colorino» genotypes by AFLP and ISTR DNA marker technology Vitis 35: 183–88.
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, and Zabeau M (1995) AFLP: a new technique for DNA finger-printing. Nucl Acids Res 23: 4407–14.
Wetton JH, Carter RE, Parkin DT, and Walters D, (1987) Demographic study of a wild House Sparrow population by DNA fingerprinting. Nature 327: 147–9.
Winter P and Kahl G (1995) Molecular marker technologies for plant improvement. World J Microbiol Biotechnol 11: 438–48.
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Demey, J.R., Gamez, E., Molina, S. et al. Comparative study of the discriminating capacity of AFLP and ISTR markers for genetic analysis ofAgave fourcroydes . Plant Mol Biol Rep 22, 29–35 (2004). https://doi.org/10.1007/BF02773346
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DOI: https://doi.org/10.1007/BF02773346