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Construction of a sugar beet BAC library from a hybrid with diverse traits

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Abstract

A bacterial artificial chromosome (BAC) library of the 750-Mbp sugar beet genome represented in hybrid US H20 was constructed fromHind III-digested DNA, with an average insert size of 120 kbp. US H20 is a variety grown in the eastern United States. It exhibits heterosis for emergence and yield, presumably because of its hybridity between eastern and western US germplasm sources. Filter arrays were used to assess the abundance and distribution of particular nucleotide sequences. An rRNA gene probe found that 1.2% of the library carried sequences similar to these highly repetitive and conserved sequences. A simple sequence repeat element (CA)8 thought to be predominantly distributed throughout centromere regions of all chromosomes was present in 1.7% of clones. For more than half of the 28 randomly chosen expressed sequence tags (ESTs) used as probes, a higher-than-expected number of single-copy hybridization signals was observed. Assuming 6× genome coverage, this suggests that many duplicate genes exist in the beet genome.

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Abbreviations

BAC:

bacterial artificial chromosome

CMS:

cytoplasmic male sterility

EST:

expressed sequence tag

SNP:

single nucleotide polymorphism

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Authors and Affiliations

  1. USDA-ARS Sugarbeet and Bean Research Unit, Department of Crop and Soil Sciences, Michigan State University, 494 Plant and Soil Sciences Building, 48824-1325, East Lansing, MI

    J. Mitchell McGrath & R. Scott Shaw

  2. Department of Biological Sciences, University of Maine, 5751 Murray Hall, 04469-5751, Orono, ME

    Benildo G. de los Reyes

  3. Northern Crop Science Lab, USDA-ARS, 1307 18th St N, 58105-5677, Fargo, ND

    John J. Weiland

Authors
  1. J. Mitchell McGrath
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  2. R. Scott Shaw
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  3. Benildo G. de los Reyes
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  4. John J. Weiland
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McGrath, J.M., Shaw, R.S., de los Reyes, B.G. et al. Construction of a sugar beet BAC library from a hybrid with diverse traits. Plant Mol Biol Rep 22, 23–28 (2004). https://doi.org/10.1007/BF02773345

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