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An easy and reliable method for establishment and maintenance of leaf and root cell cultures ofArabidopsis thaliana

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Abstract

Cell suspension cultures are useful for a wide range of biochemical and physiological studies, yet their production can be technically demanding and often unreliable. Here we describe a protocol for producing Arabidopsis cell suspension cultures that is reliable and easy to use.

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Abbreviations

BAP:

6-benzyl-aminopurine

2,4-D:

2,4-dichloro-phenoxyacetic acid

IAA:

indole-3-acetic acid

MS:

Murashige and Skoog medium (1962)

NAA:

napthaleneacetic acid

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Author information

Authors and Affiliations

  1. Experimental Station La Mayora (CSIC), 29750 Algarrobo-Costa, Malaga, Spain

    CL Encina

  2. Plant Genetic Engineering Laboratory, Department of Botany, University of Queensland, 4072, Brisbane, Australia

    M Constantin & J Botella

Authors
  1. CL Encina
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  2. M Constantin
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  3. J Botella
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Corresponding author

Correspondence to J Botella.

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Encina, C., Constantin, M. & Botella, J. An easy and reliable method for establishment and maintenance of leaf and root cell cultures ofArabidopsis thaliana . Plant Mol Biol Rep 19, 245–248 (2001). https://doi.org/10.1007/BF02772896

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  • DOI: https://doi.org/10.1007/BF02772896

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