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Stable genetic transformation of cotton plants using polybrene-spermidine treatment

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Abstract

Polybrene and/or spermidine treatments were used to deliver plasmid DNA into cotton suspension culture obtained from cotyledon-induced callus. The transforming plasmid (pBI221.23) contained the selectablehpt gene for hygromycin resistance and the screenablegus gene. Primary transformant cotton plants were regenerated and analyzed by DNA hybridization and β-glucuronidase assay. The combination polybrene-spermidine treatment greatly enhanced the uptake and expression of DNA and the recovery of nonchimeric germ-line transgenic cotton plants.

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  1. Institute of Microbiology and Genetics, Vienna Biocenter, Developmental Genetics and Plant Biotechnology Section, University of Vienna, Dr. Bohrgasse 9, A-1030, Vienna, Austria

    Wagdy A. Sawahel

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  1. Wagdy A. Sawahel
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Correspondence to Wagdy A. Sawahel.

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Sawahel, W.A. Stable genetic transformation of cotton plants using polybrene-spermidine treatment. Plant Mol Biol Rep 19, 377 (2001). https://doi.org/10.1007/BF02772838

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  • DOI: https://doi.org/10.1007/BF02772838

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