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A method of subtraction hybridization mediated by oligo(dT) particles

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Abstract

A subtraction hybridization procedure using magnetic oligo(dT) particles originally designed for mRNA isolation was developed. Magnetic particles are used to isolate mRNA, prime cDNA synthesis, and eliminate transcripts common to control and experimental samples. The protocol was utilized to isolate 2 tomato cDNAs upregulated during infection withVerticillium dahliae race II.

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Krista L. Thomas & K. Peter Pauls

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  1. Krista L. Thomas
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  2. K. Peter Pauls
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Correspondence to K. Peter Pauls.

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Thomas, K.L., Pauls, K.P. A method of subtraction hybridization mediated by oligo(dT) particles. Plant Mol Biol Rep 19, 373 (2001). https://doi.org/10.1007/BF02772836

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  • DOI: https://doi.org/10.1007/BF02772836

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