Abstract
To detect exogenous genes in genetically modified plants, we have designed a multiplex polymerase chain reaction (MPCR) system that includes 7 primer pairs. With this method, the promoter, terminator, selection gene, reporter gene, and function gene can be detected simultaneously. The concentrations of each primer were optimized according to AT/TA%. Several genes in plasmid PBI121, genetically modified tobacco, and imported soybeans were detected, and the results were consistent with standard PCR. The method was sensitive enough to detect a single copy sequence.
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Abbreviations
- 2 x MPCR:
-
MPCR with 2 primer pairs
- GMP:
-
genetically modified plant
- MPCR:
-
multiplex PCR
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Tao, Z., Cai, XF., Yang, SL. et al. Detection of exogenous genes in genetically modified plants with multiplex polymerase chain reaction. Plant Mol Biol Rep 19, 289–298 (2001). https://doi.org/10.1007/BF02772827
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DOI: https://doi.org/10.1007/BF02772827