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Modified AFLP analysis method for species with small genomes

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Abstract

A modified method for detecting amplified fragment length polymorphism (AFLP) has been developed for species with small genomes. We successfully used a combination of primers with a reduced number of selective bases to differentiate and fingerprint isolates ofColletotrichum acutatum, the causative agent of strawberry black spot, an important strawberry disease.

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Abbreviations

AFLP:

amplified fragment length polymorphism

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Authors and Affiliations

  1. Horticulture Research International, East Malling, ME19 6BJ, West Malling, Kent, UK

    Celia M. James & Graeme J. Down

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute of Fruit Breeding, Pillnitzer Plantz 3a, D-01326, Dresden, Germany

    Silke S. Lesemann

Authors
  1. Celia M. James
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  2. Silke S. Lesemann
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  3. Graeme J. Down
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Correspondence to Celia M. James.

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James, C.M., Lesemann, S.S. & Down, G.J. Modified AFLP analysis method for species with small genomes. Plant Mol Biol Rep 21, 303–307 (2003). https://doi.org/10.1007/BF02772806

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  • DOI: https://doi.org/10.1007/BF02772806

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