Abstract
We present a method for rapid isolation of flanking regions from amplified fragment length polymorphism (AFLP) fragments based on thermal asymmetric interlaced (TAIL)-PCR, in which one sequence-specific primer and one degenerate primer derived from an conserved motif found in homologies of the known sequence were used. The final result showed this to be a simple and efficient strategy, especially for short known sequences containing coding regions. Moreover this protocol was especially useful for species with little available genome information such as Hongkong Kumquat (Fortunella hindsii), since most of their genes have known homologies in other species such asArabidopsis and rice.
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Meng, H., Xu, J., Guo, W. et al. A protocol for rapid isolation of flanking regions from short known sequences. Plant Mol Biol Rep 23, 75 (2005). https://doi.org/10.1007/BF02772649
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DOI: https://doi.org/10.1007/BF02772649