Abstract
A protocol for efficient direct gene transfer by using particle gun bombardment was developed for mothbeanVigna aconitifolia L. Jacq. Marechal. Hypocotyl explants from 2 cultivars of mothbean were transformed with 3 plasmids: pBI121, pHS101, and pHS102. Stable transformants were regenerated on MS medium supplemented with benzyladenine, α-naphthaleneacetic acid, and kanamycin. The helium pressure, plasmid type, and cultivar that were used determined the stable transformation frequency. Complete plants were regenerated and transferred to soil. The integration of the stable transgenes and reporter genes in plant genomes was shown by means of PCR amplification of these genes from plant genomic DNA and Southern blot hybridization with gene-specific probes. This method allows high-efficiency production of transgenic plants in mothbean.
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Suchita Kamble and Hari S. Misra contributed equally to this work.
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Kamble, S., Misra, H.S., Mahajan, S.K. et al. A protocol for efficient biolistic transformation of mothbeanVigna aconitifolia L. Jacq. Marechal. Plant Mol Biol Rep 21, 457–458 (2003). https://doi.org/10.1007/BF02772595
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DOI: https://doi.org/10.1007/BF02772595