Abstract
Activation tagging is a powerful method of insertional mutagenesis for generating gain-of-function mutations in plants. Current activation-tagging, cassettes, based on the 35S enhancer of the cauliflower mosaic virus, have limited utility in genetic backgrounds containing 35S promoter sequences because they may cause homology-dependent gene silencing. We constructed series of novel activation-tagging vectors that do not contain the CaMV 35S enhancer but instead contain multiple tandem copies of an alternative enhancer from cassava vein mosaic virus. For selection, the T-DNAs confer Basta herbicide resistance. Resulting activation-tagging cassettes were introduced intoArabidopsis thaliana to demonstrate stable integration of the T-DNA. Vectors described here may be suitable for, but not limited to, activation-tagging projects in genetic backgrounds harboring transgenes driven by the CaMV 35S promoter.
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Abbreviations
- COP1:
-
constitutive photomorphogenesis 1
- CsVMV:
-
cassava vein mosaic virus
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Dong, Y., von Arnim, A.G. Novel plant activation-tagging vectors designed to minimize 35S enhancer-mediated gene silencing. Plant Mol Biol Rep 21, 349–358 (2003). https://doi.org/10.1007/BF02772584
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DOI: https://doi.org/10.1007/BF02772584