Abstract
The completed sequence of theArabidopsis genome shows that a single base pair deletion exists in the gene encoding the MAP kinase ATMPK5 (locus At4g11330) when compared with the cDNA. This has led to the annotation of ATMPK5 in databases as a truncated protein kinase or with a deletion in kinase subdomain I. Both situations would lead to a mutated nonfunctional protein. We amplified and sequenced the relevant region from genomic DNA of theArabidopsis ecotypes Columbia and Wassilewskija, and we found that both contain sequences that are in accordance with the cDNA sequence. We propose, therefore, that the ATMPK5 locus can encode a functional MAP kinase protein. This finding is relevant to the further functional analysis of this locus.
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Abbreviations
- MAP kinase:
-
mitogen-activated protein kinase
- ORF:
-
open reading frame
- RT-PCR:
-
reverse transcription-polymerase chain reaction
- UTR:
-
untranslated region
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Giuliani, C., Heberle-Bors, E. & Wilson, C. The At4g11330 (ATMPK5) locus ofArabidopsis thaliana: Updating the annotation. Plant Mol Biol Rep 21, 327–331 (2003). https://doi.org/10.1007/BF02772581
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DOI: https://doi.org/10.1007/BF02772581