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Improved technique for isolating RNA from tobacco tissues

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Abstract

We have developed a much-improved method for isolating RNA from tobacco tissue. The novel component of the described RNA isolation method is the addition of lithium chloride to the extraction buffer. Following that, the RNA was homogenized with phenol/chloroform and precipitated in ethanol. This isolation technique provided highly reproducible and good quality RNA within 2 h.

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Authors and Affiliations

  1. Division of Food and Biotechnology, Kangwon National University, Chuncheon, 200-701, Kangwon, Korea

    Myeong-Hyeon Wang & Hak-Ik Rhee

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  1. Myeong-Hyeon Wang
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  2. Hak-Ik Rhee
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Correspondence to Myeong-Hyeon Wang.

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Wang, MH., Rhee, HI. Improved technique for isolating RNA from tobacco tissues. Plant Mol Biol Rep 19, 187 (2001). https://doi.org/10.1007/BF02772164

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  • DOI: https://doi.org/10.1007/BF02772164

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