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A rapid PCR based method to establish the potential for paternal inheritance of chloroplasts inPelargonium

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Abstract

A simple procedure for the amplification of chloroplast DNA from pollen is described. This allows the potential for biparental or paternal inheritance of chloroplasts in angiosperms to be established rapidly and reliably. Such information is important when chloroplast DNA, which is generally assumed to be inherited maternally, is used to study gene flow or reconstruct phylogenies. The technique could also prove valuable in assessing the risks of transgene escape via pollen of genetically modified plants, in which the chloroplast genome rather than the nucleus has been transformed.

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Abbreviations

ctDNA:

chloroplast DNA

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Horticulture Research International, East Malling, ME19 6BJ, West Malling, Kent, U.K.

    Celia M. James

  2. Department of Genetics, Downing Street, CB2 3EH, Cambridge, U.K.

    John A. Barrett

  3. Department of Botany, The Natural History Museum, SW7 5BD, London, U.K.

    Stephen J. Russell

  4. Royal Botanic Garden Edinburgh, 20A Inverleith Row, EH3 5LR, Edinburgh, U.K.

    Mary Gibby

Authors
  1. Celia M. James
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  2. John A. Barrett
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  3. Stephen J. Russell
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  4. Mary Gibby
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Correspondence to Celia M. James.

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James, C.M., Barrett, J.A., Russell, S.J. et al. A rapid PCR based method to establish the potential for paternal inheritance of chloroplasts inPelargonium . Plant Mol Biol Rep 19, 163–167 (2001). https://doi.org/10.1007/BF02772159

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  • DOI: https://doi.org/10.1007/BF02772159

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