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High-throughput DNA extraction from forest trees

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Abstract

It is difficult to extract pure high-quality DNA from trees, which may not be amenable to advances in extraction methods suitable for other plants. A new commercial high-throughput DNA extraction system, using a silica binding matrix for purification and a multisample mixer mill for tissue disruption, was evaluated for its suitability withEucalyptus spp.,Pinus spp., andAraucaria cunninghamii (hoop pine). DNA suitable for a range of molecular biology applications was successfully extracted from all genera. The method was highly reliable when tested in more than 500 preparations and could be adapted to different tree species with relatively minor modifications.

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Authors and Affiliations

  1. Cooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University, Military Rd, 2480, Lismore, New South Wales, Australia

    Mervyn Shepherd, Michael Cross, Rhonda L. Stokoe & Leon J. Scott

  2. Centre for Plant Conservation Genetics, Southern Cross University, Military Rd, 2480, Lismore, New South Wales, Australia

    Megan E. Jones

Authors
  1. Mervyn Shepherd
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  2. Michael Cross
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  3. Rhonda L. Stokoe
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  4. Leon J. Scott
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  5. Megan E. Jones
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Corresponding author

Correspondence to Mervyn Shepherd.

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Shepherd, M., Cross, M., Stokoe, R.L. et al. High-throughput DNA extraction from forest trees. Plant Mol Biol Rep 20, 425 (2002). https://doi.org/10.1007/BF02772134

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  • DOI: https://doi.org/10.1007/BF02772134

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