Abstract
Using a heterologous probe, we localized thepetB andpetD genes on a 2.4-kbpBamH I fragment ofPopulus deltoides ctDNA. The fragment was cloned and characterized by means of nucleotide sequencing. Analysis of the complete nucleotide sequence of this fragment revealed the presence of a 213-codon exon exhibiting more than 90% homology with thepetB gene nucleotide sequence of higher plants. We identified a mini-exon and of thepetD gene downstream from this exon. The presence of conserved splice site sequences suggests that the poplarpetB andpetD genes contain group II introns. Furthermore, these genes constitute an operon withPSII genes,psbB, psbT, andpsbH, and cotranscribe with them as a polycistronic message. Northern hybridization analysis with total RNA prepared from leaves at different developmental stages revealed a quantitative increase in the primary transcript in mature leaves.
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Abbreviations
- MOPS:
-
3-(N-morpholino)-propane sulfonic acid
- NC:
-
nonconserved
- NS:
-
nonsynonymous substitutions
- nt:
-
nucleotide
- RBS:
-
ribosomal binding site
- S:
-
similar
- SC:
-
semiconserved
- SS:
-
synonymous substitutions
- ctDNA:
-
chloroplast DNA
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Dixit, R., Trivedi, P., Nath, P. et al. Characterization ofpetB andpetD genes of thePopulus deltoides chloroplastpsbB operon. Plant Mol Biol Rep 20, 357–368 (2002). https://doi.org/10.1007/BF02772123
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DOI: https://doi.org/10.1007/BF02772123