Since culture ofBorrelia burgdorferi from patients with chronic Lyme disease has been an extraordinarily rare event, clarification of the nature of the illness and proving its etiology as infectious have been difficult. A method for reliably and reproducibly culturingB. burgdorferi from the blood of patients with chronic Lyme disease was therefore sought by making a controlled blood culture trial studying 47 patients with chronic Lyme disease. All had relapsed after long-term oral and intravenous antibiotics. 23 patients with other chronic illness formed the control group. Positive cultures were confirmed by fluorescent antibody immuno-electron microscopy using monoclonal antibody directed against Osp A, and Osp A PCR. 43/47 patients (91%) cultured positive. 23/23 controls (100%) cultured negative. Although persistent infection has been, to date, strongly suggested in chronic Lyme disease by positive PCR and antigen capture, there are major problems with these tests. This new method for culturingsB. burgdorferi from patients with chronic Lyme disease certainly defines the nature of the illness and establishes that it is of chronic infectious etiology. This discovery should help to reestablish the gold standard in laboratory diagnosis of Lyme disease.
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Krupp, L. B., Masur, D., Schwartz, J., Coyle, P. K., Langenback, L. J., Fernquist, S. K.: Cognitive functioning in late Lyme borreliosis. Arch. Neurol. 48 (1991) 1125–1129.
Logigian, E. L., Kaplan, R. F., Steere, A. C.: Chronic neurologic manifestation of Lyme disease. N. Engl. J. Med. 323 (1990) 1438–1444.
Bayer, M. E., Zhang, L., Bayer, M. H.:Borrelia burgdorferi DNA in the urine of treated patients with chronic Lyme diseases symptoms. A PCR study of 97 cases. Infection 24 (1996) 347–353.
Nocton, J. J., Dressler, F., Rutledge, B. J., Rys, P. N., Persing, D. H., Steere, A. C.: Detection ofBorrelia burgdorferi DNA by polymerase chain reaction in synovial fluid from patients with Lyme arthritis. N. Engl. J. Med. 330 (1994) 229–234.
Preac Mursic, V., Weber, K., Pfister, H. W., Wilske, B., Gross, B., Baumann, A., Prokop, J.: Survival ofBorrelia burgdorferi in antibiotically treated patients with Lyme borreliosis. Infection 17 (1989) 355–359.
Schmidli, J., Hunzicker, T., Moesli, P., Schaad, U. B.: Cultivations ofBorrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis. J. Infect. Dis. 158 (1988) 905–906.
Pfister, H. W., Preac Mursic, V., Wilske, B., Schielke, E., Sorgel, F., Einhaupl, K. M.: Randomized comparison of ceftriaxone and cefotaxime in Lyme neuroborreliosis. J. Infect. Dis. 163 (1991) 311–318.
Hassler, D., Riedel, K., Zorn, J., Preac Mursic, V.: Pulsed high-dose cefotaxime therapy in refractory Lyme borreliosis (letter). Lancet 338 (1991) 193.
Nadelman, R. B., Pavia, C. S., Magnarelle, L. A., Wormser, G. P.: Isolation ofBorrelia burgdorferi from the blood of seven patients with Lyme disease. Am. J. Med. 88 (1990) 21–26.
Berger, B. W., Johnson, R. C., Kodner, C., Coleman, L.: Cultivation ofBorrelia burgdorferi from the blood of two patients with erythema migrans lesions lacking extracutaneous signs and symptoms of Lyme disease. J. Am. Acad. Dermatol. 30 (1994) 48–51.
Hulínská, D., Bartak, P., Hercogova, J., Hancil, J., Basta, J., Schramlova, J.: Electron microscopy of Langerhans cells andBorrelia burgdorferi in Lyme disease patients. Zbl. Bakt. 280 (1994) 348–359.
Hulínská, D., Krausova, M., Janovska, D., Rohacova, H., Hancil, J., Mailer, H.: Electron microscopy and the polymerase chain reaction of spirochetes from the blood of patients with Lyme disease. Cent. Eur. J. Public Health 1 (1993) 81–85.
Sigal, L. H., Paterlla, S. J.: Lyme arthritis as the incorrect diagnosis in pediatric and adolescent fibromyalgia. Pediatrics 90 (1992) 523–528.
Dinerman, H., Steere, A. C.: Lyme disease associated with fibromyalgia. Ann. Intern. Med. 117 (1992) 281–285.
Steere, A. C., Taylor, E., McHugh, G. L., Logigian, E. L.: The overdiagnosis of Lyme disease. JAMA 269 (1993) 1812–1816.
Schutzer, S. E., Coyle, P. K., Belman, A. L., Golightly, M. G., Drulle, J.: Sequestration of antibody toBorrelia burgdorferi in immune complexes in seronegative Lyme disease. Lancet 335 (1990) 312–315.
Preac Mursic, V., Wanner, G., Reinhardt, S., Wilske, B., Busch, U., Marget, W.: Formation and cultivation ofBorrelia burgdorferi spheroplast L-form variants. Infection 24 (1996) 218–226.
Brorson, Ø., Brorson, S. H.: Transformation of cystic forms ofBorrelia burgdorferi to normal, mobile spirochetes. Infection 25 (1997) 240–246.
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Phillips, S.E., Mattman, L.H., Hulínská, D. et al. A proposal for the reliable culture ofBorrelia burgdorferi from patients with chronic lyme disease, even from those previously aggressively treated. Infection 26, 364–367 (1998). https://doi.org/10.1007/BF02770837
- Lyme Disease
- Borrelia Burgdorferi
- Lyme Borreliosis
- Original Magnification