, 42:349 | Cite as

Toxicity of diflubenzuron, pyriproxyfen, imidacloprid and diafenthiuron to the predatory bugOrius laevigatus (Het.: Anthocoridae)

  • F. Delbeke
  • P. Vercruysse
  • L. Tirry
  • P. De Clercq
  • D. Degheele


The susceptibility of the predatory bugOrius laevigatus (Fieber) to the insect growth regulators diflubenzuron, pyriproxyfen, the nitroguanidine insecticide imidacloprid and the thiourea compound diafenthiuron was investigated in the laboratory. Fifth-instar nymphs were exposed to formulated materials of each compound and adults were exposed to formulated materials of diafenthiuron and imidacloprid. In each case, exposure via ingestion and residual contact was tested. Pyriproxyfen was harmless toO. laevigatus nymphs by both ways of exposure. The respective LC50-values of diflubenzuron via ingestion and residual contact were 229.9 and 391.1 mg a.i./l. Diafenthiuron did not cause significant mortality to fifth-instar nymphs and adults via ingestion but was toxic by residual contact with LC50-values of 329.4 mg a.i./l and 125.9 mg a.i./l for nymphs and adults respectively. Imidacloprid proved to be the most toxic compound with LC50-values of 1.1 and 0.04 mg a.i./l for nymphs and 2.1 and 0.3 mg a.i./l for adults, via ingestion and residual contact, respectively. The results suggest that use of pyriproxyfen in an integrated pest management programme will not cause any problems but that imidacloprid, and to a lesser extent, also diflubenzuron and diafenthiuron could be harmful to the predator.


Orius laevigatus diflubenzuron pyriproxyfen imidacloprid diafenthiruon side-effects 

La toxicité du diflubenzuron, pyriproxyfen, imidacloprid et diafenthiuron sur le prédateurOrius laevigatus (Fieber) (Heteroptera : Anthocoridae)


La sensibilité du prédateurO. laevigatus envers les insecticides diflubenzuron, pyriproxyfen, imidacloprid et diafenthiuron a été déterminée au laboratoire. Les larves du cinquième stade ont été exposées aux matériaux formulés de chaque produit et les adultes ont été exposés aux matériaux formulés de diafenthiuron et imidacloprid. Dans tous les cas, le traitement par ingestion et par contact résiduel a été examiné.

Le pyriproxyfen s’est avéré sans danger pour les larves deO. laevigatus selon les deux manières d’exposition. Les valeurs CL50 du diflubenzuron par ingestion et contact résiduel étaient de 229.9 et 39.1 mg m.a./l., respectivement. Le diafenthiuron n’a pas provoqué de mortalité significative par ingestion chez les larves et les adults. Par contre, le diafenthiuron s’est montré toxique par contact résiduel, avec des valeurs CL50 de 329.4 et 125.9 mg m.a./l pour les larves et les adultes, respectivement. L’imidacloprid était le produit le plus toxique avec des valeurs CL50 DE 1.1 ET 0.04 mg m.a./l pour les larves et 2.1 et 0.3 mg m.a./l pour les adultes, par ingestion et par contact résiduel, respectivement.

Les données suggèrent que l’usage du pyriproxyfen ne causera pas de problèmes dans un programme de lutte intégrée. Cependant l’usage de l’imidacloprid et, à un degré moindre, du diflubenzuron et du diafenthiuron en combinaison avecO. laevigatus doit être considéré avec prudence.


  1. Anonymous. — 1989. Polo (diafenthiuron, CGA 106630), Technical data sheet. — Ciba-Geigy, Basel, Switzerland.Google Scholar
  2. Anonymous. — 1994. The pesticide manual. Tenth edition,Crop Protection Publications, 1 341 p.Google Scholar
  3. Askari, A. &Stern, V. M. — 1972. Effect of temperature and photoperiod onOrius tristicolor feeding onTetranychus pacificus. —J. Econ. Entomol., 65, 132–135.Google Scholar
  4. Broadbent, A. B. &Pree, D. J. — 1984. Effects of diflubenzuron and BAY SIR 8514 on beneficial insects associated with peach. —Environ. Entomol., 13, 133–136.Google Scholar
  5. Brodsgaard, H. F. — 1989. Coloured sticky traps forFrankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in glasshouses. —J. Appl. Entomol., 107, 136–140.CrossRefGoogle Scholar
  6. Celli, G. &Nicoli, G. — 1989. Calcium and barium polysulphides reassessed. —Informatore Agrario, 45, 97–98.Google Scholar
  7. Clement, P. &David, B. V. — 1988. Evaluation of some new insecticides against pests and their effects on mites in okra. —Current Research, University of Agricultural Science Bangalore, 15, 132–133.Google Scholar
  8. De Clercq, P., De Cock, A., Tirry, L., Vinuela, E. &Degheele, D. — 1995. Toxicity of diflubenzuron and pyriproxyfen to the predatory bugPodisus maculiventris. —Entomol. Exp. Appl., 74, 17–22.CrossRefGoogle Scholar
  9. De Cock A., De Clercq, P., Tirry, L. &Degheele, D. — 1996. Toxicity of diafenthiuron and imidacloprid to the predatory bugPodisus maculiventris (Heteroptera: Pentatomidae). —Environ. Entomol., 25, 476–480.Google Scholar
  10. Elbert, A., Becker, B., Hartwig, J. &Erdelen, C. — 1991. Imidacloprid — a enw systemic insecticide. —Pflanzensch. Nachr. Bayer, 44, 113–135.Google Scholar
  11. El-Sayad, A. E. G. H. &El-Ghar, G. E. S. A. — 1992. The influence of normal and low-rate application of insecticides on populations of the cotton whitefly and melon aphid and associated parasites and predators on cucumber. —Anz. Schädlingsk. Pflanzensch. Umweltsch., 65, 54–57.CrossRefGoogle Scholar
  12. Fayad, Y. H. &Ibrahim, A. A. — 1988. Impact of successive insecticidal applications at different interval periods on the number of predators in cotton fields. —Bull. Entomol. Soc. Egypt, 15, 47–48.Google Scholar
  13. Grosscurt, A. C. — 1978. Diflubenzuron: some aspects of its ovicidal and larvicidal mode of action and an evaluation of its practical possibilities. —Pestic. Sci., 9, 373–383.CrossRefGoogle Scholar
  14. Hatakoshi, M., Nagui, N. &Nakayama, I. — 1986). 2-(1-Methyl-2-(4-phenoxyphenoxy)ethoxy) pyridine as a new insect juvenile hormone analogue: induction of supernumerary larvae inSpodoptera litura (Lepidoptera: Noctuidae). —Appl. Entomol. Zool., 21, 351–353.Google Scholar
  15. Hattingh, V. &Tate, B. — 1995. Effects of field-weathered residues of insect growth regulators on some Coccinellidae (Coleoptera) of economic importance as biocontrol agents. —Bull. Entomol. Res., 85, 489–493.CrossRefGoogle Scholar
  16. Helyer, N. L. — 1991. Laboratory pesticides screening method for aphid predatory midgeAphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae). —Biocontrol Sci. Technol, 1, 53–58.Google Scholar
  17. Hough-Goldstein, J. &Whalen, J. — 1993. Inundative release of predatory stink bugs for control of Colorado potato beetle. —Biol. Control, 3, 343–347.CrossRefGoogle Scholar
  18. Ishaaya, I., Mendelson, Z. &Horowitz, A. R. — 1993. Toxicity and growth suppression exerted by diafenthiuron in the sweetpotato whitefly,Bemisia tabaci. —Phytoparasitica, 21, 199–204.Google Scholar
  19. Kadir, H. A. &Knowles, C. O. — 1991. Toxicological studies of the thiourea diafenthiuron on diamondback moths (Lepidoptera: Yponomeutidae), twospotted spider mites (Acari: Tetranychidae), and bulb mites (Acari: Acaridae). —J. Econ. Entomol., 84, 780–784.Google Scholar
  20. Kawada, H., Dohara, K. &Shinjo, G. — 1987. Evaluation of larvicidal potency of insect growth regulator 2-(1-methyl-2-(4-phenoxyphenoxy)ethoxy)pyridine against the housefly,Musca domestica. —Jap. J. Sanit. Zool., 38, 317–322.Google Scholar
  21. Kawashima, K. — 1988. Effects of chitin synthesis inhibitors on the four-spotted lacewingChrysopa septempunctata. —Annual report of the Society of Plant Protection of North Japan, 39, 246–247.Google Scholar
  22. Langley, P. A., Felton, T. &Oouchi, H. — 1988. Juvenile hormone mimics as effective sterilants for the tsetse flyGlossina morsitans morsitans. —Med. Vet. Entomol., 2, 29–35.PubMedGoogle Scholar
  23. Langley, P. A., Howl, V. &Oouchi, H. — 1990. Regulation of reproduction inRhodnius prolixus by the juvenile hormone mimic pyriproxyfen. —Entomol. Exp. Appl., 57, 271–279.CrossRefGoogle Scholar
  24. Leicht, W. — 1993. Imidacloprid — a chloronicotinyl insecticide. —Pestic. Outlook, 5, 17–21.Google Scholar
  25. LeOra Software — 1987. POLO-PC: a user’s guide to probit or logit analyses. —Berkeley, CA.Google Scholar
  26. Mattioli, E., Carvalho, C. F. &Salgado, L. O. — 1992. Effects of insecticides and acaricides on eggs, larvae and adults of predaceousCeraeochiysa cubana (Hagen) (Neuroptera: Chrysopidae) under laboratory. —Ciencia e Pratica, 16, 491–497.Google Scholar
  27. Mizell, R. F. &Sconyers, M. C. — 1992. Toxicity of imidacloprid to selected arthropod predators in the laboratory. —Fla. Entomol., 75, 277–280.CrossRefGoogle Scholar
  28. Mullins, J. W. — 1993. Imidacloprid. A new nitroguanidine insecticide. —ACS Symposium series, 524, 183–198.CrossRefGoogle Scholar
  29. Nagai, K. — 1990a. Effects of a juvenile hormone mimic material, 4-phenoxyphenyl (RS)-2(2-pyridoxyl) propyl ether, onThrips palmi Karny (Thysanoptera: Thripidae) and its predatorOrius spp. (Hemiptera: Anthocoridae). —Appl. Entomol. Zool., 25, 199–204.Google Scholar
  30. Nagai, K. — 1990b. Effects of insecticides onOrius spp., the natural enemy ofThrips palmi Karny. —Jap. J. Appl. Entomol. Zool., 34, 321–324.Google Scholar
  31. Nagai, K. — 1990c. Suppressive effect ofOrius sp. (Hemiptera: Anthocoridae) on the population density ofThrips palmi (Thysanoptera: Thripidae) on eggplant in an open field. —Jap. J. Appl. Entomol. Zool., 34, 109–114.Google Scholar
  32. Nagai, K. — 1991. Integrated control programs forThrips palmi (Karny) on eggplants (Solanum melongena L.) in an open field. —Jap. J. Appl. Entomol. Zool., 34, 283–289.Google Scholar
  33. Oetting, R. — 1990. Imidacloprid for the control of whitefliesTrialeurodes vaporariorum andBemisia tabaci in greenhouse poinsettias. —Brighton Crop Protection Conference — Pests and Diseases (Brighton, UK), 2, 367–372.Google Scholar
  34. Peleg, B. A. — 1988. Effect of a new phenoxy juvenile hormone analog on California red scale (Homoptera: Diaspididae), Florida was scale (Homoptera: Coccidae) and ectoparasiteAphytis holoxanthus DeBache (Hymenoptera: Aphelinidae). —J. Econ. Entomol., 81, 88–92.Google Scholar
  35. Pflüger, W. &Schmuck, R. — 1991. Ecotoxicological profile of imidacloprid. —Pflanzensch. Nachr. Bayer, 44, 145–158.Google Scholar
  36. Retnakaran, A. &Wright, J. E. — 1987. Control of insect pests with benzoylphenyl ureas. In: Chitin and benzoylphenyl ureas (J. E. Wright &A. Retnakaran, eds.). —Dr W. Junk Publ., Dordrecht: pp. 205–288.Google Scholar
  37. Robb, K. L., Parrella, M. P. &Newman, J. P. — 1988. The biology and control of the Western flower thrips. Part 1. —Ohio’s Florists’ Association, 699, 2–5.Google Scholar
  38. Ruder, F.J. &Kayser, H. — 1993. The carbodiimide product of diafenthiuron inhibits mitochondria in vivo. —Pestic. Biochem. Physiol., 46, 96–106.CrossRefGoogle Scholar
  39. Sauphanor, B., Chabrol, L., Faivre D’Arcier, F., Sureau, F. &Lenfant, C. — 1993. Side effects of diflubenzuron on a pear psylla predator:Forficula auricularia. —Entomophaga, 38, 163–174.CrossRefGoogle Scholar
  40. Soltani, N. — 1983. Effects of ingested diflubenzuron on the longevity and the peritrophic membrane of adult mealworms (Tenebrio molitor L.). —Pestic. Sci., 15, 221–225.CrossRefGoogle Scholar
  41. Streibert, H. P., Drabek, J. &Rindlisbacher, A. — 1988. CGA 160630 — a new type of acaricide/insecticide for the control of sucking pests complex in cotton and other crops. —Brighton Crop Protection Conference — Pests and Diseases (Brighton, UK), 1, 25–33.Google Scholar
  42. Van de Veire, M. — 1995. Integrated pest management in glasshouse tomatoes, sweet peppers and cucumbers in Belgium. — Doctoral dissertation, University of Ghent, 133 pp.Google Scholar
  43. Van de Veire, M. &Degheele, D. — 1992. Biological control of the western flower thrips,Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), in glasshouse sweet peppers withOrius spp. (Heteroptera: Anthocoridae). A comparative study betweenO. niger (Wolff) andO. insidiosus (Say). —Biocontrol Sci. Technol., 2, 281–283.CrossRefGoogle Scholar
  44. Van De Veire, M. &Degheele, D. — 1993. Side effects of diafenthiuron on the greenhouse whitefly parasitoidEncarsia formosa and the predatory bugOrius niger and its possible use in IPM in greenhouse vegetables. —Med Fac. Landbouww., Univ. Gent, 53, 509–514.Google Scholar
  45. Van Laecke, K. &Degheele, D. — 1993. Effect of insecticide-synergist combinations on the survival ofSpodoptera exigua. —Pestic. Sci., 37, 283–288.CrossRefGoogle Scholar
  46. Vercruysse, P. — 1996. Neveneffekten van imidacloprid en diafenthiuron opOrius laevigatus (Fieber). — M. S. thesis, University of Gent, 108 pp.Google Scholar
  47. Vogt, H. — 1992. Investigations on the side-effects of insecticides and acaricides onChrysoperla carnea Steph. (Neuroptera: Chrysopidae). —Med. Fac. Landbouww., Univ. Gent, 57, 559–567.Google Scholar
  48. Wilkinson, J. D., Biever, K. D., Ignoffo, C. M., Pons, W. J., Morrison, R. K. &Seay, R. S. — 1978. Evaluation of diflubenzuron formulations on selected insect parasitoids and predators. —J. Georgia Entomol. Soc., 13, 227–236.Google Scholar
  49. Woodford, J. A. T. &Mann, J. A. — 1992. Systemic effects of imidacloprid on aphid feeding behaviour and virus transmission on potatoes. —Brighton Crop Protection Conference — Pests and Diseases (Brighton, UK), 3, 557–563.Google Scholar
  50. Yakti, R. &Poehling, H. M. — 1988. Zum Einfluss eines Insektenwachstumsregulators (DSC 24 300 I) auf die Entwicklung vonAphis fabae anVicia faba unter besonderer Berücksichtigung von Nebenwirkungen auf Blattlauspredatoren. —Med. Fac. Landbouww., Univ. Gent, 53, 1033–1043.Google Scholar
  51. Yokoyama, V. Y. &Miller, G. T. — 1991. Potential of pyriproxyfen as a quarantine treatment for codling moth and oriental fruit moth (Lepidoptera: Tortricidae). —J. Econ. Entomol., 84, 942–947.Google Scholar

Copyright information

© Lavoisier Abonnements 1997

Authors and Affiliations

  • F. Delbeke
    • 1
  • P. Vercruysse
    • 1
  • L. Tirry
    • 1
  • P. De Clercq
    • 1
  • D. Degheele
    • 1
  1. 1.Laboratory of Agrozoology, Faculty of Agricultural & Applied Biological SciencesUniversity of GentGentBelgium

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