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Detectionin situ of endophyte withinTricholoma matsutake/Pinus densiflora mycorrhizal roots

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Tricholoma matsutake/Pinus densiflora mycorrhizas andP. densiflora roots were collected from beneath matureT. matsutake fruit-bodies in a Shiro in central Japan and investigated for evidence of endophytic infection.Tricholoma matsutake infection was determined microscopically on cleared, bleached and stained mycorrhizas by the presence of both a thin, discontinuous mantle and highly branched, sparingly septate Hartig net mycelium within the root cortex. Endophytic infection of Matsutake mycorrhizas was characterized by intracortical sclerotia, simple septate mycelium within the cortex and vascular cylinder and intracellular spore masses within the root cortex.

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Corresponding author

Correspondence to Warwick M. Gill.

Additional information

This research was supported by a grant from the Bio-oriented Technology Research Advancement Institution (BRAIN).

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Gill, W.M., Suzuki, K. Detectionin situ of endophyte withinTricholoma matsutake/Pinus densiflora mycorrhizal roots. J For Res 5, 289 (2000).

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Key words

  • ectomycorrhiza
  • endophyte
  • Pinus densiflora
  • Tricholoma matsutake