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Modulation of cardiac interstitial noradrenaline levels through KATP channels during ischemic preconditioning in rabbits: comparison of the effect of anesthesia between pentobarbital and ketamine + xylazine

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Summary

In rabbits, both the stimulation of α1-adrenoceptors and ischemic preconditioning (PC) reduce infarct size. One candidate for the mechanism of PC is noradrenaline (NA), which stimulates α1-adrenoceptors in the myocardium during PC. Opening of the KATP channel is considered to be another candidate for PC, since a KATP channel blocker, glibenclamide, blocks the infarct size-reducing effect of the PC of 5-min ischemia and 5-min reperfusion in rabbits anesthetized with ketamine + xylazine. However, in rabbits anesthetized with pentobarbital, the infarct size-reducing effect of PC was not blocked by glibenclamide. The effect of glibenclamide on the PC effect thus differs depending on the anesthesia used. Therefore, we speculated that the increase in cardiac interstitial NA levels induced by PC may be modified by the anesthesia used, thus regulating the effect of glibenclamide on the PC effect. In open-chest Japanese white male rabbits anesthetized with pentobarbital or ketamine + xylazine, myocardial interstitial NA levels were measured before and during the PC of 5-min ischemia and 5-min reperfusion in the presence or absence of the KATP channel blocker, glibenclamide (0.3mg/kg, i.v.), using a microdialysis technique. The NA levels were measured using high-performance liquid chromatography coupled with electrochemical detection. The PC of 5-min ischemia and 5-min reperfusion significantly elevated the interstitial NA level. This increase in the NA level was not blocked by glibenclamide under anesthesia with pentobarbital. Under anesthesia with ketamine + xylazine, the PC did not cause an increase in the myocardial interstitial NA level in either the absence or the presence of glibenclamide. In conclusion, PC elevates the myocardial interstitial NA level, and this elevation is not mediated through the opening of the KATP channel under anesthesia with pentobarbital. Under anesthesia with ketamine + xylazine, PC does not cause an increase in the myocardial interstitial NA level. This may explain the discrepancy in the blocking effect of glibenclamide on the infarct size-reducing effect of PC between anesthesia with pentobarbital and ketamine + xylazine.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Gifu University School of Medicine, 40 Tsukasa Machi, 500, Gifu, Japan

    Shinya Minatoguchi, Tatsuya Kariya, Yoshihiro Uno, Masazumi Arai, Michiya Ohno, Kazuaki Hashimoto, Yoshio Nishida, Der-Jinn Wo & Hisayoshi Fujiwara

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  1. Shinya Minatoguchi
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  2. Tatsuya Kariya
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  3. Yoshihiro Uno
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  4. Masazumi Arai
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  5. Michiya Ohno
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  6. Kazuaki Hashimoto
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  7. Yoshio Nishida
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  8. Der-Jinn Wo
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  9. Hisayoshi Fujiwara
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Minatoguchi, S., Kariya, T., Uno, Y. et al. Modulation of cardiac interstitial noradrenaline levels through KATP channels during ischemic preconditioning in rabbits: comparison of the effect of anesthesia between pentobarbital and ketamine + xylazine. Heart Vessels 12, 294–299 (1997). https://doi.org/10.1007/BF02766806

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  • DOI: https://doi.org/10.1007/BF02766806

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