Comparison of different fluorometers (PAM, 1- Hz, BackScat and Turner) measuring either chlorophyll concentration or photosynthetic activity

Vergleich unterschiedlicher fluorometer (PAM-, 1 Hz-, BackScat- und Turner-) die entweder die chlo-rophyll-konzentration oder die photosynthetische aktivitÄt messe

Summary

Like other commercial fluorometers the 1-Hz fluorometer is a device that can measure chlorophyll-α concentrations even in highly diluted phytoplankton suspensions. In contrast to those fluorometers, it measures rates of photosynthetic activity in relation to the total amount of detectable fluorescent material.

Applications include the determination of total algal pigment concentrations as well as algal productivity. In this work the primary production was detected by fluorescence yield measurements at different irradiances. Fluorometrically analysed rates were correlated with the carbon fixation rate measured by14C incubation. Fluorescence data were sampled in parallel experiments by thePAM (pulse amplitude modulated)-and 1-Hz fluorometer. The results of the14C incubation method were highly correlated with the calculated 1-Hz — andPAM fluorometer data. The applications presented show that it is possible with both fluorometers to measure the impact of environmental changes on the production of phytoplankton communitiesin vivo.

Zusammenfassung

Ähnlich anderen kommerziellen Fluorometern ist das 1Hz-Fluorometer ein Me\gerÄt, das Chlorophyll-a Konzentrationen selbst in hochverdünnten Phytoplankton-Lösungen messen kann. Im Gegensatz zu diesen ist es in der Lage, den photosynthetisch aktiven Anteil im VerhÄltnis zur Gesamt-Chlorophyll-a Fluoreszenz festzustellen.

Anwendungen dieses GerÄtes schliessen die Bestimmung des gesamten Algenpigmentgehaltes sowie die Messung der AlgenproduktivitÄt ein. Die PrimÄrproduktionsrate wurde über den Fluoreszenzyield bei unterschiedlichen LichtintensitÄten bestimmt. Sie wurde mit der Kohlenstoffaufnahme der Algen, gemessen über die14C Assimilation, korreliert. Die Messungen wurden in Parallelexperimenten mit demPAM (Pulse Amplitude Modulated)- und 1Hz-Fluorometer durchgeführt. Die Ergebnisse zeigen eine sehr gute Korrelation der14C Daten mit den berechneten 1Hz-Fluorometer- undPAM Fluorometer-Werten. Die hier gegebenen Anwendungen zeigen die Möglichkeit, mit beiden Fluorometern den Einfluss von UmweltÄnderungen auf Phytoplankton-Gemeinschaftenin vivo messen zu können.

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References

  1. Colijn, F., G. W. Kraay, R. N. M. Duin, U. Tillmann andM. J. W. Veldhuis, 1996: Design and tests of a novel Pmax incubator to be used for measuring the phytoplankton primary production in ICES monitoring studies. Report of the ICES WG on Phytoplankton Ecology, Reykjavik 1996.

  2. Dau, H., 1994: Short-term adaptation of plants to changing irradiances and its relation to Photosystem II photochemistry and fluorescence emission.J. Photochem. Photobiol.26, 3–27.

    Article  Google Scholar 

  3. Genty, B., J.-M. Briantais andN. R. Baker, 1989: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.Biochim. Biophys. Acta990, 87–92.

    Google Scholar 

  4. Guillard, R. R. L. andJ. H. Ryther, 1962. Studies on marine planktonic diatoms.I. Cyclotella nana Hustedt andDetonula confervacea (Cleve) —Gran. Can. J. Microbiol.8, 229–239.

    Google Scholar 

  5. Hartig, P. andF. Colijn, 1996: Pulse-Amplitude-Modulation-Fluorescence(PAM) — a tool for fast assessment of primary productivity in the sea? In: Integrated Marine System Analysis, Baeyens J. et al. (ed.), Vrije Universiteit Brussel, 103–121.

  6. Hofstraat, J. W., C. H. Peeters, J. F. H. Snel andC. Geel, 1994: Simple determination of photosynthetic efficiency and photoinhibition ofDunaliella tertiolecta by saturating pulse fluorescence measurements.Mar. Ecol. Prog. Ser.103, 187–196.

    Article  Google Scholar 

  7. Lorenzen, C. J., 1967: Determination of chlorophyll and pheo-pigments: spectrophotometric equations.Limnol. Oceanogr.12, 343–346.

    Article  Google Scholar 

  8. Mantoura, R. F. C. andC. A. Llewellyn, 1983: The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography.Analyt. Chim. Acta151, 297–314.

    Article  Google Scholar 

  9. Moldaenke, C., K. H. Vanselow andU.-P. Hansen, 1995: The 1-Hz fluorometer: A new approach to fast and sensitive long-term studies of active chlorophyll and environmental influences.Helgol. Meeresunters.49, 785–796.

    Article  Google Scholar 

  10. Schreiber, U., 1994: New Emitter-Detector-Cuvette assembly for measuring modulated chlorophyll fluorescence of high diluted suspensions in conjunction with the standardPAM fluorometer.Z Naturforsch.49c, 646–656.

    Google Scholar 

  11. van Kooten, O. andJ. F. H. Snel, 1990: The use of chlorophyll fluorescence nomenclature in plant stress physiology.Photosynth. Res.25, 147–150.

    Article  Google Scholar 

  12. Vanselow, K. H., 1993: The effect of N-nutrients on the acceptor pool of PSI and thylakoid energization as measured by chlorophyll fluorescence ofDunaliella salina.J. Exp. Bot.44, 1331–1340.

    Article  Google Scholar 

  13. Vanselow, K. H. undU.-P. Hansen, 1989: Möglichkeiten eines Biotests mit Hilfe der linearen Analyse der Kinetik der Signale aus dem photosynthetischen Apparat III. Off-line Analyse der Chlorophyll-Fluoreszenzkinetik mit rauschigem Licht. In: Grundlagen und Anwendungsbereiche der Chlorophyll-Fluoreszenz, (Herausg. Ernst, D., Schmidt, Chr.,) Dortmunder BeitrÄge zur Wasserforschung 37: 135–151.

  14. Vanselow, K. H., J. Kolbowski andU.-P. Hansen, 1989: Analysis of chlorophyll-fluorescence by means of noisy light.J. Exp. Bot.40, 247–256.

    Article  Google Scholar 

  15. Vanselow, K. H., Hansen, U.-P., B. Ruesch undCh. Moldaenke, 1992: Anwendung von Rauschanalyse und Doppelkorrelator in der vko-Technik. In: 7. Symposium Maritime Elektronik (Herausg. H. Albrecht and O. Wild) Univ. Rostock, Rostock 39: 44–47.

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Correspondence to Klaus Heinrich Vanselow.

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Vanselow, K.H., Hintze, R., Hartig, P. et al. Comparison of different fluorometers (PAM, 1- Hz, BackScat and Turner) measuring either chlorophyll concentration or photosynthetic activity. Deutsche Hydrographische Zeitschrift 49, 375–384 (1997). https://doi.org/10.1007/BF02764046

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Keywords

  • Phytoplankton
  • Chlorophyll Fluorescence
  • Carbon Fixation
  • Fluorescence Yield
  • Dunaliella