Comparison of different fluorometers (PAM, 1- Hz, BackScat and Turner) measuring either chlorophyll concentration or photosynthetic activity

Vergleich unterschiedlicher fluorometer (PAM-, 1 Hz-, BackScat- und Turner-) die entweder die chlo-rophyll-konzentration oder die photosynthetische aktivitÄt messe


Like other commercial fluorometers the 1-Hz fluorometer is a device that can measure chlorophyll-α concentrations even in highly diluted phytoplankton suspensions. In contrast to those fluorometers, it measures rates of photosynthetic activity in relation to the total amount of detectable fluorescent material.

Applications include the determination of total algal pigment concentrations as well as algal productivity. In this work the primary production was detected by fluorescence yield measurements at different irradiances. Fluorometrically analysed rates were correlated with the carbon fixation rate measured by14C incubation. Fluorescence data were sampled in parallel experiments by thePAM (pulse amplitude modulated)-and 1-Hz fluorometer. The results of the14C incubation method were highly correlated with the calculated 1-Hz — andPAM fluorometer data. The applications presented show that it is possible with both fluorometers to measure the impact of environmental changes on the production of phytoplankton communitiesin vivo.


Ähnlich anderen kommerziellen Fluorometern ist das 1Hz-Fluorometer ein Me\gerÄt, das Chlorophyll-a Konzentrationen selbst in hochverdünnten Phytoplankton-Lösungen messen kann. Im Gegensatz zu diesen ist es in der Lage, den photosynthetisch aktiven Anteil im VerhÄltnis zur Gesamt-Chlorophyll-a Fluoreszenz festzustellen.

Anwendungen dieses GerÄtes schliessen die Bestimmung des gesamten Algenpigmentgehaltes sowie die Messung der AlgenproduktivitÄt ein. Die PrimÄrproduktionsrate wurde über den Fluoreszenzyield bei unterschiedlichen LichtintensitÄten bestimmt. Sie wurde mit der Kohlenstoffaufnahme der Algen, gemessen über die14C Assimilation, korreliert. Die Messungen wurden in Parallelexperimenten mit demPAM (Pulse Amplitude Modulated)- und 1Hz-Fluorometer durchgeführt. Die Ergebnisse zeigen eine sehr gute Korrelation der14C Daten mit den berechneten 1Hz-Fluorometer- undPAM Fluorometer-Werten. Die hier gegebenen Anwendungen zeigen die Möglichkeit, mit beiden Fluorometern den Einfluss von UmweltÄnderungen auf Phytoplankton-Gemeinschaftenin vivo messen zu können.

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Vanselow, K.H., Hintze, R., Hartig, P. et al. Comparison of different fluorometers (PAM, 1- Hz, BackScat and Turner) measuring either chlorophyll concentration or photosynthetic activity. Deutsche Hydrographische Zeitschrift 49, 375–384 (1997).

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  • Phytoplankton
  • Chlorophyll Fluorescence
  • Carbon Fixation
  • Fluorescence Yield
  • Dunaliella