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Rapid PCR amplification of chimeric products and its direct application to in vitro testing of recombinant DNA construction strategies

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An Erratum to this article was published on 01 October 1997

Abstract

This article describes a simple and rapid method for efficient production of chimeric products by polymerase chain reaction (PCR). This protocol is amenable to site-directed mutagenesis strategies and can be done without the time-consuming gel purification step. The PCR products generated can also be directly used for direct gene transfer into plant cells without further subcloning to test construction strategies.

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Authors and Affiliations

  1. Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA

    Myeong-Je Cho & Peggy G. Lemaux

Authors
  1. Myeong-Je Cho
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  2. Peggy G. Lemaux
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Correspondence to Myeong-Je Cho.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02752269.

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Cho, MJ., Lemaux, P.G. Rapid PCR amplification of chimeric products and its direct application to in vitro testing of recombinant DNA construction strategies. Mol Biotechnol 8, 13–16 (1997). https://doi.org/10.1007/BF02762336

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  • DOI: https://doi.org/10.1007/BF02762336

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