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A simple and rapid method for preparing genomic DNA from gram-positive bacteria

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Abstract

Molecular epidemiologic and other studies may require preparation of genomic DNA from large numbers of bacteria in sufficiently pure form for restriction endonuclease digestion, cloning, RAPD-PCR, Southern hybridization, and so on.Staphylococcus and other Gram-positive bacteria have a rigid cell wall and can be difficult to lyse. Here, a simple and rapid method for the preparation of genomic DNA from multiple samples is reported. This method produces clean DNA for use in most molecular biology methods in <90 min.

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Authors and Affiliations

  1. Biochemistry Department, University of Oxford, South Parks Road, OX1 3QU, Oxford, England, UK

    Muhammad Sohail

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  1. Muhammad Sohail
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Correspondence to Muhammad Sohail.

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Sohail, M. A simple and rapid method for preparing genomic DNA from gram-positive bacteria. Mol Biotechnol 10, 191–193 (1998). https://doi.org/10.1007/BF02760866

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  • DOI: https://doi.org/10.1007/BF02760866

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