Abstract
Fourteen peptides corresponding to sequences of all the exposed and some of the transmembrane protein regions of porin A from the outer membrane ofNeisseria meningitidis strain B:15:P1.7,16 were synthesized. Mice of various lines were immunized with the free peptides not conjugated with any protein carrier. It was shown that the majority of the peptides possess immunogenic properties. Two peptides were identified binding to antibodies present in the serum of mice after meningitis. Protective properties of a number of the synthesized peptides were studied, and three peptide sequences inducing mice protection to an experimental infection withN. meningitidis were identified.
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Abbreviations
- DIEA:
-
diisopropylethylamine
- DIPC:
-
N,N′-diisopropylcarbodiimide
- DMAP:
-
4-(dimethylamino)pyridine
- DMS:
-
dimethyl sulphide
- Fmoc:
-
9-fluorenylmethoxycarbonyl
- HOBI:
-
1-hydroxybenzotriazole
- Pbf:
-
2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl
- PBS:
-
0.01 M phosphate-buffered saline (pH 7.4)
- PorA:
-
porin A
- TBTU:
-
2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate
References
Frasch, C.E.,Clin. Microbiol. Rev., 1989, vol. 2, pp. 134–138.
Gotschlich, E.C., Goldchneider, J., and Artenstein, S.,J. Exp. Med., 1969, vol. 129, pp. 1367–1384.
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Koroev, D.O., Kotelnikova, O.V., Volpina, O.M. et al. Induction of antimeningitis immunity by the synthetic peptides: I. The immunoactive synthetic fragments of porin A fromNeisseria meningitidis . Russ J Bioorg Chem 26, 291–296 (2000). https://doi.org/10.1007/BF02759280
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DOI: https://doi.org/10.1007/BF02759280