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An express method for testing the activity of a repair enzyme, uracil-DNA-glycosylase


A rapid and effective method for testing a repair enzyme, uracil-DNA-glycosylase, was proposed. As a substrate, a deoxyuridine-containing 5′-32P-labeled deoxyoligonucleotide covalently attached to a polystyrene support (Tenta Gel S-NH2) was used. The ammonia cleavage of the apyrimidine site formed in the enzymic reaction followed by the transition of the labeled oligonucleotide fragment from the solid phase into solution allowed the detection of the enzymic activity.

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apyrimidine/apurine site


oligonucleotide containing [3H]-deoxyuridine




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Correspondence to E. A. Kubareva.

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Sud’ina, A.E., Volkov, E.M., Oretskaya, T.S. et al. An express method for testing the activity of a repair enzyme, uracil-DNA-glycosylase. Russ J Bioorg Chem 26, 398–402 (2000).

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Key words

  • apyrimidine site
  • enzymic activity
  • repair enzyme
  • uracil-DNA-glycosylase