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The solid phase coupling of peptide segments catalyzed by the subtilisin-sodium dodecyl sulfate complex

Russian Journal of Bioorganic Chemistry volume 26, pages 369–374 (2000)Cite this article

Abstract

The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic medium. By a two-stage enzymic condensation of peptide fragments on aminosilochrom (A) containing Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It was shown that the condensation products can be split off from the support using Met residue cleavage by BrCN.

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Abbreviations

Hse:

homoseryl residue

pNA:

p-nitroanilide

SDS:

sodium dodecyl sulfate

SPS:

solid phase peptide synthesis

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Authors and Affiliations

  1. Chemical Department, Moscow State University, 119899, Vorob’evy gory, Moscow, Russia

    S. V. Kolobanova, I. Yu. Filippova, E. N. Lysogorskaya, I. V. Getun, A. V. Bacheva, E. S. Oksenoit & V. M. Stepanov

Authors
  1. S. V. Kolobanova
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  2. I. Yu. Filippova
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  3. E. N. Lysogorskaya
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  4. I. V. Getun
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  5. A. V. Bacheva
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  6. E. S. Oksenoit
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  7. V. M. Stepanov
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Correspondence to I. Yu. Filippova.

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Deceased.

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Kolobanova, S.V., Filippova, I.Y., Lysogorskaya, E.N. et al. The solid phase coupling of peptide segments catalyzed by the subtilisin-sodium dodecyl sulfate complex. Russ J Bioorg Chem 26, 369–374 (2000). https://doi.org/10.1007/BF02758663

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  • DOI: https://doi.org/10.1007/BF02758663

Key words

  • enzymic peptide synthesis
  • solid support
  • SDS-subtilisin