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The turbidimetric study of latex particle agglutination: Assay of 2,4-dichlorophenoxyacetic acid


By the example of 2,4-dichlorophenoxyacetic acid (2,4-D) assay by the inhibition of latex agglutination, new synthetic polymer microspheres for the conjugation with antibodies to 2,4-D and agglutinators based on ovalbumin and polyacrylamide were developed and characterized. The effect of various parameters (the concentration of reagents, the type and the degree of modification of the microsphere surface, and the nature of the carrier in the composition of the agglutinator) on the rate of agglutination and the maximal optical absorption observed during the reaction were studied by turbidimetry. The optimal parameters were found for the assay of 2,4-D by the inhibition of latex agglutination with turbidimetric registration of the results.

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6-aminocaproic acid


2,4-dichlorophenoxyacetic acid


copolymer microspheres

MS-Ab and MS-Aca-Ab:

MS conjugates with Ab



2,4-D-OVA and 2,4-D-PAA:

agglutinators on the basis of ovalbumin and polyacrylamide, respectively

ΔA max :

the maximal value of optical absorption; and ΔA/min, the rate of the reaction of latex agglutination


  1. Paul, W.,Fundamental Immunology, Paul, W., Ed., New York: Raven Press Books, 1984. Translated under the titleImmunologiya, Moscow: Mir, 1987, pp. 14–45,

    Google Scholar 

  2. Bangs, L.B.,Uniform Latex Particles, Indianapolis: Seradyn, 1987.

    Google Scholar 

  3. Kitano, H., Iwai, S., Okubo, T., and Ise, N.,J. Am. Chem. Soc., 1987, vol. 109, pp. 7609–7612.

    Google Scholar 

  4. Thompson, J.C., Craig, A.R., Davey, C.L., Newman, D.J., Lonsdale, M.L., Bucher, W.J., Nagle, P.D., and Price, C.P.,Clin. Chem. (Washington, D.C.), 1997, vol. 43, pp. 2384–2389.

    CAS  Google Scholar 

  5. Lukin, Yu.V., Dokuchaev, I.M., Polyak, I.M., and Eremin, S.A.,Anal. Lett., 1994, vol. 27, pp. 2973–2982.

    CAS  Google Scholar 

  6. Lukin, Yu.V., Generalova, A.N., Tyrtysh, T.V., and Eremin, S.A.,Immunochemical Technology for Environmental Applications, Aga, D.S. and Thurman, E.M., Eds., Washington: Am. Chem. Soc., ACS Symposium Series 657, 1997, pp. 97–105.

    Google Scholar 

  7. Van der Hul, H.J. and Vanderhoff, J.W.,Br. Polym. J., 1970, vol. 2, pp. 121–127.

    Article  Google Scholar 

  8. Montagne, P., Omari, R.E., Cliquet, F., Cuiliere, M.L., and Duheille, J.,Bioconjugate Chem., 1992, vol. 3, pp. 504–509.

    Article  CAS  Google Scholar 

  9. Gritskova, I.A., Sedakova, L.I., Muradyan, D.S., Sinekaev, B.M., Pavlov, A.V., and Pravednikov, A.N.,Dokl. Akad. Nauk SSSR, 1978, vol. 243, p. 403.

    CAS  Google Scholar 

  10. Lunskaya, I.A., Eremin, S.A., Egorov, A.M., Kolar, V., and Franek, M.,Agrokhimiya, 1993, no. 2, pp. 113–118.

  11. Margel, S.,J. Polym. Sci., 1984, vol. 22, pp. 3521–3533.

    CAS  Google Scholar 

  12. Eremin, S.A., Lunskaya, I.A., Samsonova, Zh.V., and Egorov, A.M.,Bioorg. Khim., 1993, vol. 19, pp. 836–844.

    CAS  Google Scholar 

  13. Ivanov, A.E., Belov, S.V., and Zubov, V.P.,J. Polym. Sci., 1993, vol. 35, pp. 1093–1098.

    Google Scholar 

  14. Bovin, N.V., Korchagina, E.Yu., Zemlyanukhina, T.V., Byramova, N.E., Galanina, O.E., Zemlyakov, A.E., Ivanov, A.E., Zubov, V.P., and Mochalova, L.V.,Glycoconjugate J., 1993, vol. 10, pp. 142–151.

    Article  CAS  Google Scholar 

  15. Kondo, A., Kawano, T., and Higashitani, K.,J. Ferment. Bioeng., 1992, vol. 73, pp. 435–439.

    Article  CAS  Google Scholar 

  16. Prakticheskaya khimiya belka (Practical Chemistry of Proteins), Moscow: Mir, 1989, p. 293.

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Correspondence to V. P. Zubov.

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Generalova, A.N., Buryakov, A.N., Lukin, Y.V. et al. The turbidimetric study of latex particle agglutination: Assay of 2,4-dichlorophenoxyacetic acid. Russ J Bioorg Chem 26, 490–495 (2000).

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Key words

  • agglutinator
  • 2,4-dichlorophenoxyacetic acid
  • hapten
  • latex particle agglutination
  • polymer microspheres
  • turbidimetric analysis