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The turbidimetric study of latex particle agglutination: Assay of 2,4-dichlorophenoxyacetic acid

Abstract

By the example of 2,4-dichlorophenoxyacetic acid (2,4-D) assay by the inhibition of latex agglutination, new synthetic polymer microspheres for the conjugation with antibodies to 2,4-D and agglutinators based on ovalbumin and polyacrylamide were developed and characterized. The effect of various parameters (the concentration of reagents, the type and the degree of modification of the microsphere surface, and the nature of the carrier in the composition of the agglutinator) on the rate of agglutination and the maximal optical absorption observed during the reaction were studied by turbidimetry. The optimal parameters were found for the assay of 2,4-D by the inhibition of latex agglutination with turbidimetric registration of the results.

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Abbreviations

Ab:

antibodies

Aca:

6-aminocaproic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

copolymer microspheres

MS-Ab and MS-Aca-Ab:

MS conjugates with Ab

OVA:

ovalbumin

2,4-D-OVA and 2,4-D-PAA:

agglutinators on the basis of ovalbumin and polyacrylamide, respectively

ΔA max :

the maximal value of optical absorption; and ΔA/min, the rate of the reaction of latex agglutination

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Correspondence to V. P. Zubov.

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Generalova, A.N., Buryakov, A.N., Lukin, Y.V. et al. The turbidimetric study of latex particle agglutination: Assay of 2,4-dichlorophenoxyacetic acid. Russ J Bioorg Chem 26, 490–495 (2000). https://doi.org/10.1007/BF02758620

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  • DOI: https://doi.org/10.1007/BF02758620

Key words

  • agglutinator
  • 2,4-dichlorophenoxyacetic acid
  • hapten
  • latex particle agglutination
  • polymer microspheres
  • turbidimetric analysis