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A biologically active fragment of the differentiation factor of the HL-60 line cells: Identification and properties

Abstract

Six-membered peptide fragment TGENHR (HLDF-6) was identified in the HL-60 cell culture of human promyelocyte leukemia treated with retinoic acid when studying the differentiation factor HLDF of this cell line. HLDF-6 retains the ability of the full-size factor to induce the differentiation and arrest the proliferation of the starting HL-60 cells. It was shown that the synthetic peptide HLDF-6 has no specific receptors on the surface of the HL-60 cells but can affect the binding of interleukin IL-1β, a cytokine involved in proliferation, to the cell surface. It was found on a model of transplantable NSO myeloma that HLDF-6 has an antitumor activity.

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Abbreviations

Dns:

dansyl

HEPES:

N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

HLDF (Human Leukemia Differentiation Factor):

a differentiation factor of the cell line HL-60 of human promyelocyte leukemia

NBT:

Nitro Blue Tetrazolium dye

PMSF:

phenylmethylsulfonyl fluoride

rHulL-1β:

human recombinant interleukin-1β

rHuIFN-α2:

human recombinant interferon-α2

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Correspondence to I. A. Kostanyan.

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Kostanyan, I.A., Astapova, M.V., Navolotskaya, E.V. et al. A biologically active fragment of the differentiation factor of the HL-60 line cells: Identification and properties. Russ J Bioorg Chem 26, 450–456 (2000). https://doi.org/10.1007/BF02758614

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  • DOI: https://doi.org/10.1007/BF02758614

Key words

  • cell differentiation factor
  • biologically active fragment
  • peptide
  • HL-60 cell line