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A biologically active fragment of the differentiation factor of the HL-60 line cells: Identification and properties

Russian Journal of Bioorganic Chemistry volume 26, pages 450–456 (2000)Cite this article

Abstract

Six-membered peptide fragment TGENHR (HLDF-6) was identified in the HL-60 cell culture of human promyelocyte leukemia treated with retinoic acid when studying the differentiation factor HLDF of this cell line. HLDF-6 retains the ability of the full-size factor to induce the differentiation and arrest the proliferation of the starting HL-60 cells. It was shown that the synthetic peptide HLDF-6 has no specific receptors on the surface of the HL-60 cells but can affect the binding of interleukin IL-1β, a cytokine involved in proliferation, to the cell surface. It was found on a model of transplantable NSO myeloma that HLDF-6 has an antitumor activity.

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Abbreviations

Dns:

dansyl

HEPES:

N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

HLDF (Human Leukemia Differentiation Factor):

a differentiation factor of the cell line HL-60 of human promyelocyte leukemia

NBT:

Nitro Blue Tetrazolium dye

PMSF:

phenylmethylsulfonyl fluoride

rHulL-1β:

human recombinant interleukin-1β

rHuIFN-α2:

human recombinant interferon-α2

References

  1. Kostanyan, I.A., Astapova, M.V., Starovoytova, E.V., Dranitsina, S.M., and Lipkin, V.M.,FEBS Lett., 1994, vol. 356, pp. 327–329.

    PubMed  Article  CAS  Google Scholar 

  2. Smirnova, E.V., Rakitina, T.V., Evtodienko, A. Yu., Kostanyan, I.A., and Lipkin, V.M.,Bioorg. Khim., 2000, vol. 26, pp. 392–396.

    PubMed  CAS  Google Scholar 

  3. Dranitsyna, S.M., Kostanyan, I.A., Andreeva, S.G., Astapova, M.V., Babichenko, I.I., Baeva, O.V., Bogachuk, A.P., Molotkovskaya, I.M., Rodionov, I.L., Smirnova, E.V., and Lipkin, V.M.,Bioorg. Khim., 2000, vol. 26, pp. 340–351.

    PubMed  CAS  Google Scholar 

  4. Baehner, R.L. and Nathan, D.G.,J. Med. Chem., 1968, vol. 278, pp. 971–976.

    CAS  Google Scholar 

  5. Lakowicz, J.R.,Principles of Fluorescence Spectroscopy, New York: Plenum, 1984, pp. 122–155.

    Google Scholar 

  6. Dinarello, C.A.,Blood, 1996, vol. 87, pp. 2095–2147.

    PubMed  CAS  Google Scholar 

  7. Novick, D., Cohen, B., and Rubinstein, M.,Cell, 1994, vol. 77, pp. 391–400.

    PubMed  Article  CAS  Google Scholar 

  8. Leftwich, J.A. and Hall, R.E.,Cancer Res., 1989, vol. 49, pp. 4459–4465.

    PubMed  CAS  Google Scholar 

  9. Leftwich, J.A. and Hall, R.E.,J. Leukocyte Biol., 1990, vol. 47, pp. 87–96.

    PubMed  CAS  Google Scholar 

  10. Hall, R.E., Agarwal, S., Kestler, D.P., Cobb, J.A., Goldstein, K.M., and Chang, N.-S.,Biochem. J., 1996, vol. 319, pp. 919–927.

    PubMed  CAS  Google Scholar 

  11. Kestler, D.P., Agarwal, S., and Hall, R.E.,Immunology, 1995, vol. 86, pp. 463–468.

    PubMed  CAS  Google Scholar 

  12. Hermanek, P.,Pathol. Res. Pract., 1994, vol. 190, pp. 97–102.

    PubMed  CAS  Google Scholar 

  13. Akaza, H.,Nippon Hinyokika Gakkai Zasshi, 1994, vol. 85, pp. 229–241.

    PubMed  CAS  Google Scholar 

  14. Rodionov, I., Baru, M., and Ivanov, V.,Pept. Res., 1992, vol. 5, pp. 119–125.

    PubMed  CAS  Google Scholar 

  15. Tesenkov, V.,Reaktsiya blast-transformatsii limfotsitov selezenki pod vliyaniem antigena in vitro. Immunologicheskie metody (The Blastotransformation of Spleen Lymphocytes under the Action of an Antigenin vitro in:Immunological Methods), Frimel’, Kh., Ed., Moscow: Mir, 1979, pp. 80–92.

    Google Scholar 

  16. Gray, R.,Methods Enzymol., 1967, vol. 11, pp. 138–151.

    Google Scholar 

  17. Zolotarev, Yu.A., Nezavibatko, V.N., Borisov, Yu.A., Rosenberg, S.G., and Myasoedov, N.F.,Amino Acids, 1993, vol. 5, pp. 121–122.

    Google Scholar 

  18. Chang, K.J., Jacobs, S., and Cuatrecasas, P.,Biochim. Biophys. Acta, 1975, vol. 406, pp. 294–303.

    PubMed  Article  CAS  Google Scholar 

  19. Salacinski, P.R.P., McLean, C., Sykes, J.E.C., Clement-Jones, V.V., and Lowry, P.,Anal. Biochem., 1981, vol. 117, pp. 136–146.

    PubMed  Article  CAS  Google Scholar 

  20. Holtman, H. and Wallach, D.,J. Immunol., 1987, vol. 139, pp. 1161–1167.

    Google Scholar 

  21. Liposomes: A Practical Approach, New, R.R.C., Ed., Oxford: Oxford Univ., 1990, pp. 52–56.

    Google Scholar 

  22. Yelton, D.E., Diamond, B.A., Kwan, S.P., and Scharff, M.D.,Curr. Top. Microbiol. Immunol., 1978, vol. 81, pp. 1–7.

    PubMed  CAS  Google Scholar 

  23. Galfre, G. and Milstein, C., Preparation of Monoclonal Antibodies: Strategies and Procedures,Methods Enzymol., Chapter I, Langohe, I.I. and van Vunakies, H., Eds., Orlando: Academic, 1981, p. 73.

    Google Scholar 

  24. Histopathologic Technique and Practical Histochemistry, Lillie, R.D., Ed., London: Academic, 1965, pp. 166–167.

    Google Scholar 

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871, GSP-7 Moscow, Russia

    I. A. Kostanyan, M. V. Astapova, S. M. Dranitsyna, I. M. Molotkovskaya & V. M. Lipkin

  2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    E. V. Navolotskaya, T. N. Lepikhova, G. B. Telegin, I. L. Rodionov & L. K. Baidakova

  3. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow

    Yu. A. Zolotarev

Authors
  1. I. A. Kostanyan
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  2. M. V. Astapova
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  3. E. V. Navolotskaya
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  4. T. N. Lepikhova
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  5. S. M. Dranitsyna
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  6. G. B. Telegin
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  7. I. L. Rodionov
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  8. L. K. Baidakova
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  9. Yu. A. Zolotarev
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  10. I. M. Molotkovskaya
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  11. V. M. Lipkin
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Correspondence to I. A. Kostanyan.

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Kostanyan, I.A., Astapova, M.V., Navolotskaya, E.V. et al. A biologically active fragment of the differentiation factor of the HL-60 line cells: Identification and properties. Russ J Bioorg Chem 26, 450–456 (2000). https://doi.org/10.1007/BF02758614

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  • DOI: https://doi.org/10.1007/BF02758614

Key words

  • cell differentiation factor
  • biologically active fragment
  • peptide
  • HL-60 cell line