Abstract
The initial stages of colonization of wheat roots by cells ofAzospirillum brasilense strains 75 and 80 isolated from soils of the Saratov oblast were studied. The adsorption of azospirilla on root hairs of soft spring wheats rapidly increased in the first hours of incubation, going then to a plateau phase. Within the first 15 h of incubation, exponential-phase cells were adsorbed more intensively than stationary-phase cells. Conversely, stationary-phase cells were adsorbed more intensively than exponential-phase cells, if the period of azospirilla incubation with the wheat roots was extended. As the time of incubation increased, the attachment of azospirilla to the wheat roots became stronger. The effect of cell attachment to root hairs was strain-dependent; the number of adsorbed cells of a given strain of azospirilla was greater in the case of host wheat cultivars. The deformation of wheat root hairs was affected by the polysaccharide-containing complexes isolated from the capsular material of azospirilla. The suggestion is made that common receptor systems are involved in the adsorption of azospirilla on roots and in root hair deformation
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bashan, Y. and Holguin, G.,Azospirillum-Plant Relationships: Environmental and Physiological Advances (1990–1996),Can. J. Microbiol., 1997, vol. 43, pp. 103–121.
Levanony, H., Bashin, Y., Romano, B., and Klein, E., Ultrastructural Localization and Identification ofAzospirillum brasilense Cd on and within Wheat Root by Immunogold Labelling,Plant Soil, 1989, vol. 117, no. 2, pp. 207–218.
Okon, Y. and Kapulnik, Y., Development and Function of Azospirillum-inoculated Roots,Plant Soil, 1986, vol. 90, pp. 3–16.
Zamudio, M. and Bastarrachea, F., Adhesiveness and Root Hair Deformation Capacityof Azospirillum Strains for Wheat Seedlings,Soil Biol. Biochem., 1994, vol. 26, no. 6, pp. 791–797.
Pinheiro, R.O., Baldani, J.I., and Boddey, R.M., Specificity in the Adsorption of Strains ofAzospirillum spp. to Wheat Roots,9th Int. Congr. on Nitrogen Fixation, Cancun, 1992, p. 157.
Michiels, K.W., Croes, C.L., and Van der Leyden, J., Two Different Modes of Attachment ofAzospirillum brasilense Sp7 To Wheat Roots,J. Gen. Microbiol., 1991, vol. 137, no. 9, pp. 2241–2246.
Bashan, Y., Levanony, H., and Klein, E., Evidence for a Weak Active External Adsorption ofAzospirillum brasilense Cd to Wheat Roots,J. Gen. Microbiol., 1986, vol. 132, pp. 3069–3073.
Bashan, Y. and Levanony, H., Factors Affecting the Adsorption ofAzospirillum brasilense Cd to Root Hairs as Compared with the Root Surface of Wheat,Can. J. Microbiol., 1989, vol. 35, no. 10, pp. 936–944.
Kapulnik, Y., Okon, Y., and Henis, Y., Changes in Root Morphology of Wheat Caused byAzospirillum Inoculation,Can. J. Microbiol, 1985, vol. 31, no. 10, pp. 881–887.
Konnova, S.A., Skvortsova, I.M., Makarov, O.E., Prokhorova, R.N., Rogova, T.A., and Ignatov, V.V., Polysaccharide Complexes Secreted byAzospirillum brasilense and Their Possible Role in the Interaction of Bacteria with Wheat Roots,Mikrobiologiya, 1995, vol. 64, no. 6, pp. 762–768.
Pozdnyakova, L.I., Konevskaya, S.V., Levanova, G.F., Barysheva, N.N., Pilipenko, T.Yu., Bogatyrev, V.A., and Fedorova, L.S., A Taxonomic Study of Azospirilla Isolated from Cereals Grown in the Saratov Oblast,Mikrobiologiya, 1988, vol. 57, no. 2, pp. 275–278.
Konnova, S.A., Makarov, O.E., Skvortsov, I.M., et al., Isolation, Fractionation, and Some Properties of Polysaccharides Produced in a Bound Form byAzospirillum brasilense and Their Possible Involvement in Azospirillum-Wheat Root Interactions,FEMS Microbiol. Lett., 1994, vol. 118, pp. 93–99.
Rokitskii, P.F.,Biologicheskaya statistika (Biological Statistics), Minsk: Vysheishaya Shkola, 1973.
Bashan, Y. and Holguin, G., Anchoringof Azospirillum brasilense to Hydrophobic Polystyrene and Wheat Roots,J. Gen. Microbiol, 1993, vol. 139, no. 2, pp. 379385.
Semak, N.N., Matveev, V.Yu., Panasenko, V.I., and Kotusov, V.V., Dependence of the Agglutination ofAzospirillum brasilense Sp7 by Wheat Lectin on the Culture Growth Phase,Prikl. Biokhim. Mikrobiol, 1986, vol. 22, no. 3, pp. 396–399.
Haegi, A. and Del Gallo, M., Azospirillum-Plant Interaction: A Biochemical Approach,Nitrogen Fixation, Polsinelli, M., Materassi, R., and Vincenzini, M., Eds., Dordrecht: Kluwer Academic, 1991, pp. 147–153.
Umali-Garcia, M., Hubbell, D.H., Gaskins, M.H., and Dazzo, F.B., Association ofAzospirillum with Grass Roots,Appl. Environ. Microbiol., 1980, vol. 39, no. 1, pp. 219–226.
Dazzo, F.B., Lectins and Their Saccharide Receptors as Determinants of Specificity in the Rhizobium-Legume Symbiosis,The Cell Surface: Mediator of Developmental Processes. The 38th Symposium of the Society for Developmental Biology, Subtelny, S. and Wessels, N.K., Eds., Academic, 1980, pp. 277–304.
Jain, D.K. and Patriquin, D.G., Root Hair Deformation, Bacterial Attachment, and Plant Growth in WheatAzospirillum Associations,Appl. Environ. Microbiol., 1984, vol. 48, no. 6, pp. 1208–1213.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Egorenkova, I.V., Konnova, S.A., Skvortsov, I.M. et al. Investigation of the initial stages of interaction of the bacteriumAzospirillum brasilense with wheat seedling roots: Adsorption and root hair deformation. Microbiology 69, 103–108 (2000). https://doi.org/10.1007/BF02757265
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02757265