Abstract
The study was undertaken to elucidate how different methods of storage (immersing in mineral oil, lyophilization, and subculturing) of lactic acid bacteria belonging to the generaLactobacillus andLactococcus affect their viability, antibiotic activity, and ability to accumulate organic acids. Storage of the lactic acid bacteriumLactococcus lactis subsp.lactis by immersion in mineral oil proved to be ineffective. Lyophilization allowed the survival of a sufficiently large number of cells, although their antibiotic activity somewhat decreased. The resuscitation of lyophilized bacteria by subculturing them in rich nutrient media, such as skim milk, led to the restoration of their physiological activity, including the effective antimicrobial spectrum
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Kvasnikov, E.I. and Nesterenko, O.A.,Molochnokislye bakterii i ikh ispol’zovanie (Lactic Acid Bacteria and Their Use), Moscow: Nauka, 1975, pp. 23, 290–359.
Klaenhammer, T.R., Genetics of Bacteriocins Produced by Lactic Acid Bacteria,FEMS Micwbiol. Rev., 1993, no. 12, pp. 39–86.
Bekker, M.E., Damberg, B.E., and Rappoport, A.I.,Anabioz mikroorganizmov (Microbial Anabiosis), Riga: Zinatne, 1981, p. 253.
Sidyakina, T.M., Conservation of Microorganisms in Culture Collections,Konservatsiya geneticheskikh resursov. Metody. Problemy. Perspektivy (Conservation of Gene Resources: Methods, Problems, and Prospects), Pushchino, 1991, pp. 81–159.
Arkad’eva, Z.A., Methods of Microbial Cultures Storage,Metabolizm mikroorganizmov (Microbial Metabolism), Egorov, N.S., Ed., Moscow: Mosk. Gos. Univ., 1986, pp. 57–64.
Kupletskaya, M.B. and Arkad’eva, Z.A., Long-Term Maintenance of Microorganisms in the Culture Collection of the Microbiology Department of the Moscow State University,Mikrobiologiya, 1997, vol. 66, no. 2, pp. 283–288.
Andreeva, E.F., Tarasova, I.I., and Pravdin, I.P., Some Peculiarities of the Organization of the Process of Dehydration by Sublimation under Sterile Conditions,Oborudovanie i sredstva upravleniya mikrobiologicheskikh proizvodstv (Equipment in the Microbiological Industry), 1989, vol. 2, ch. 1, pp. 148–162.
Trenina, G.A., The Central Collection of Industrial Microorganisms, Its Functions and Tasks,Biotekhnologiya, 1985, no. 1, pp. 30–39.
Oparin, Yu.G., Damage to and Protection of Biomaterials during Freezing and Lyophilization,Biotekhnologiya, 1996, no. 7, pp. 3–13.
Rappoport, A.I. and Bekker, M.E., The Effect of Sucrose and Lactose on the Resistance ofSaccharomyces cerevisiae to Dehydration,Mikrobiologiya, 1983, vol. 52, no. 5, pp. 719–722.
Inikhov, G.S. and Brio, N.P.,Metody analiza moloka i molochnykh produktov (Methods for the Analysis of Milk and Dairy Products), Moscow: Pishcheprom, 1971, pp. 234–240.
Tahara, T., Oshimura, M., and Kanatani, K., Mode of Action of Acidocin 8912, a Bacteriocin Produced byLactobacillus acidophilus TK 8912,Lett. Appl. Microbiol., 1996, vol. 23, no. 3, pp. 192–194.
Ratrikova, I.A., Gavrilova, N.N., and Kolokova, N.N., Identification of the Antibiotic Compounds Produced by Lactic Acid Bacteria,Biotekhnologiya, 1995, no. 5–6, pp. 19–20.
Vademuthu, E.B., Henderson, J.T., and Vandenbergh, P.A., Multiple Bacteriocin ProducingLactococcus and Composition, US Patent, 1994, no. 5, 348,881, C1.C12N l/12,435/252.3;435/252.4.
Egorov, N.S.,Osnovy ucheniya ob antibiotikakh (Fundamentals of the Science of Antibiotics), Moscow: Mosk. Gos. Univ., 1994, pp. 199–201.
Mitic, S. and Cuperlovic, M., Uniticai liofolizacije na transformaciju i digestibinost aminoxiseli na simbiozebacterijaStreptococcus thermophilus iLactobacillus bulgaricus, Hrana Ishrana, 1989, vol. 30, no. 1, pp. 35–36.
Mil’ko, E.S. and Egorov, N.S., The Effect of Physicochemical Parameters of the Medium on the Growth of Dissociants of Some Gram-Positive Bacteria,Biol. Nauki, 1992, no. 5, pp. 89–96.
Demina, N.S. and Lysenko, S.V., The Effect of Dehydration on the Content of Nucleic Acids and Mutational Alterations in Microorganisms,Biol. Nauki, 1989, no. 5, pp. 87–95.
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Stoyanova, L.G., Arkad’eva, Z.A. Comparative investigation of different methods of storage of lactic acid bacteria. Microbiology 69, 83–88 (2000). https://doi.org/10.1007/BF02757262
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DOI: https://doi.org/10.1007/BF02757262