Abstract
The hypothesis that various extracellular enzymes produced by the yeastSaccharomyces cerevisiae exert a mutual influence on their secretion into the cultivation medium was tested experimentally. The statistically processed results indicate that extracellular invertase affects the secretion of acid phosphatase, and acid phosphatase affects the secretion of invertase. In addition, the secretion of each of these enzymes was shown to be subject to autoregulation.
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Kaiser, C.A., Gimeno, R.E., and Shaywitz, D.A., Protein Secretion, Membrane Biogenesis, and Endocytosis,The Molecular and Cellular Biology of the Yeast Saccharomyces, Cold Spring Harbor Lab., 1997, pp. 91–228.
Yurkevich, V.V. and Kozyreva, G.T., Involvement of an Active Enzyme Protein in the Regulation of Its Formation,Dokl. Akad. Nauk SSSR, 1967, vol. 117, pp. 240–242.
Egorov, N.S., Buyak, L.I., Landau, N.S., and Al’-Nuri, M.A., Some Aspects of the Regulation of the Synthesis of Exoproteases in Associative Fungal Cultures,Nauchn. Dokl. Vyssh. Shk., Biol. Nauki, 1978, vol. 5, pp. 100–105.
Ezhov, V.V., Bezborodova, S.I., and Santsevich, N.I., On the Regulation of the Biosynthesis of Extracellular Phosphohydrolases inPenicillium brevicompactum, Mikrobiologiya, 1978, vol. 47, pp. 665–671.
Yurkevich, V.V., Regulation of the Production of a Secretable Enzyme by Its Concentration in the Medium,Usp. Sovretn. Biol., 1974, vol. 77, pp. 360–368.
Yurkevich, V.V. and Kozyreva, G.T., On the Role of the Cell Envelope in the Regulation of the Biosynthesis of α-Amylase inAspergillus oryzae, Dokl. Akad. Nauk SSSR, 1972, vol. 204, pp. 740–743.
Yurkevich, V.V. and Galcheva-Gargova, Z.V., The Role of the Cell Surface in the Regulation of the Production of β-Fructosidase in Yeasts,Zh. Obshch. Biol., 1975, vol. 36, pp. 419–422.
Karpova, O.V., Kovaleva, N.S., and Yurkevich, V.V., The Role of the Carbohydrate Component of the East Extracellular Invertase in the Regulation of the Biosynthesis of This Enzyme,Dokl Akad. Nauk SSSR, 1981, vol. 258, pp. 1009–1013.
Belousova, L.L., Secretion of Acid Phosphatase ofSaccharomyces cerevisiae under Conditions of Expression of Various Structural Genes in Immobilized Cells,Cand. Sci. (Biol.) Dissertation, Moscow, 1990.
Torriani, A.-M., Influence of Inorganic Phosphate on the Formation of Phosphatases byEscherichia coli, Biochim.Biophys. Acta, 1960, vol. 38, pp. 460–479.
Miller, G.L., Use of Dinitrosalicylic Acid Reagent for Detection of Reducing Sugars,Anal. Chem., 1959, vol. 31, pp. 426–428.
Bradford, M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilising the Principle of Protein-Dye Binding,Anal. Biochem., 1976, vol. 7, pp. 2554–2562.
Panusz, H.T., Graczyka, G., Wilmanska, D., and Scarzynska, D., Analysis of Orthophosphate-Pyrophosphate Mixture Resulting from Weak Pyrophosphatase Activities,Anal. Biochem., 1970, vol. 35, pp. 404–407.
Markov, E.V. and Lisenkov, A.N.,Planirovanie eksperimenta v usloviyakh neodnorodnostei (Planning of Experiments under Conditions of Heterogeneity), Moscow: Nauka, 1973, pp. 126–131.
Semenova, I.N., Egorov, S.N., and Egorov, N.S., Production of Secretable Acid Phosphatase during Growth ofSaccharomyces cerevisiae on Various Sources of Carbon and Nitrogen,Mikrobiologiya, 1986, vol. 65, no. 5, pp. 796–799.
Ouchterlony, O.,Handbook of Experimental Immunology, Weir, D.M., Ed., Oxford: Blackwell Sci., 1967, pp. 655–706.
Shnyreva, M.G., Tsuprun, V.L., Stel’mashuk, V.Ya., and Egorov, S.N., Analysis of the Quaternary Structure of Secretable Repressible Acid Phosphatase ofSaccharomyces cerevisiae, Biokhimiya, 1992, vol. 57, pp. 1110–1118.
Tammi, M., Ballou, L., Taylor, A., and Ballou, C.E., Effect of Glycosylation on Yeast Invertase Oligomer Stability,J. Biol. Chem., 1987, vol. 262, pp. 4395–4401.
Pryer, N.K., Wuestehube, L.J., and Schekman, R., Vesicle-mediated Protein Sorting,Annu. Rev. Biochem., 1992, vol. 64, pp. 471–516.
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Egorov, S.N., Semenova, I.N. & Maksimov, V.N. Mutual influence of invertase and acid phosphatase of the yeastSaccharomyces cerevisiae on their secretion into the cultivation medium. Microbiology 69, 26–29 (2000). https://doi.org/10.1007/BF02757252
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DOI: https://doi.org/10.1007/BF02757252