Abstract
Glutamate oxidase activity was studied in 1254Streptomyces strains isolated from the zonal soils of various regions of Russia and other countries. Seven strains proved to be producers of extracellular L-glutamate oxidase. The most active producer strain was identified, and the conditions of enzyme biosynthesis were optimized. A multistep mutagenesis-selection procedure allowed a genetically stable strain,Streptomyces sp. Z-11-6, to be obtained, whose glutamate oxidase activity was 40 times higher than that of the original natural isolate.
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Sukhacheva, M.V., Netrusov, A.I. Streptomyces sp. Z-11-6, a novel producer of extracellular L-glutamate oxidase. Microbiology 69, 13–16 (2000). https://doi.org/10.1007/BF02757249
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DOI: https://doi.org/10.1007/BF02757249