Abstract
The reaction of hemagglutination with trypsin-treated rabbit erythrocytes was used to reveal lectins on the cell surface of methanotrophic bacteria and in their culture liquids. By this method, no lectins were detected on the cell surface ofMethylococcus capsulatus IMV B-3001 andMethylomonas rubra IMV B-3075 or in the culture liquid of any of the species studied. With intact cells ofMethylocystis parvus IMV B-3491, the positive hemagglutination reaction observed was nonspecific and most probably occurred due to the high cell surface hydrophobicity characteristic of this species.
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References
Podgorskii, V.S., Kovalenko, E.L., and Simonenko, I.A.,Lektiny bakterii (Bacterial Lectins), Kiev: Naukova Dumka, 1992.
Lutsik, M.D., Panasyuk, E.N., and Lutsik, A.D.,Lektiny (Lectins), L’vov: Vyshcha Shkola, 1981.
Clyde, W.A. and Hu, PC, Antigenic Determinant of the Attachment Proteins ofM. pneumoniae Shared by Other PathogenicMycoplasma Species,Infect. Immun., 1986, vol. 51, pp. 690–692.
Karpunina, L.V., Vishnevskaya, O.A., Bogatyrev, V.A., Nikitina, V.E., and Ital’yanskaya, Yu.V., Localization of Lectins and Agglutinins in Nitrogen-fixing Soil Bacteria,Mikrobiologiya, 1995, vol. 64, no. 4, pp. 453–457.
Ivanov, M.V., Nesterov, A.I., Namsaraev, B.B., Gal’chenko, V.F., and Nazarenko, A.V., Distribution and Geochemical Activity of Methanotrophic Bacteria in Coal Mines,Mikrobiologiya, 1978, vol. 47, no. 3, pp. 489–494.
Gal’chenko, V.F., Abramochkina, F.N., Bezrukova, L.V., Sokolova, E.N., and Ivanov, M.V., Species Composition of the Aerobic Methanotrophic Microflora of the Black Sea,Mikrobiologiya, 1988, vol. 57, no. 2, pp. 305–311.
Kurdish, I.K., Roi, A.A., Koval’, E.Z., and Antonyuk, T.S., Microflora of Drifts of Western Donbas Coal Mines,Mikrobiol. Zk., 1998, vol. 60, no. 1, pp. 3–10.
Dworkin, M. and Foster, J.W., Studies ofPseudomonas methanica Sohngen nov. comb.,J. Bacteriol., 1956, vol. 72, no. 5, pp. 646–659.
Leadbetter, E.R. and Foster, J.W., Studies on Some Methane-utilizing Bacteria,Arch. Microbioi, 1958, vol. 30, no. 1, pp. 91–118.
Rosenberg, M., Gutnick, D., and Rosenberg, E., Adherence of Bacteria to Hydrocarbons: A Simple Method for Measuring Cell-Surface Hydrophobicity,FEMS Microbioi. Lett., 1980, vol. 9, no. 1, pp. 23–29.
Buchs, J., Mozes, N., Wandrey, C, and Rouxhent, P., Cell Adsorption Control by Culture Conditions: Influence of Phosphate on Surface Properties, Flocculation and Adsorption Behaviour ofCorynebacterium glutamicum, Appl. Microbioi. Biotechnol., 1988, vol. 29, no. 2/3, pp. 119–128.
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Kurdish, I.K., Roi, A.A. On the hemagglutination reaction in methanotrophic bacteria. Microbiology 69, 335–337 (2000). https://doi.org/10.1007/BF02756743
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DOI: https://doi.org/10.1007/BF02756743