Abstract
An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent arePhenol,Isoamyl alcohol,Guanidinium isothiocyanate, andBeta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained by TRIzol, an expensive but widely used monophasic reagent available commercially. The complete composition and method of preparation of PIG-B is provided to aid preparation of the reagent in the laboratory.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1982)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Gold Spring Harbor, NY.
Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Anal. Biochem. 162, 156–159.
Chomczynski, P. (1993) A reagent for the singlestep simultaneous isolation of RNA, DNA, and proteins from cell and tissue samples.Biotechniques 15, 532–536.
Sarkar, G. and Bolander, M. E. (1994) The “Looped Oligo” method for generating reference molecules for quantitative PCR.Biotechniques 17, 864–866.
Sarkar, G. and Sommer, S. S. (1989) Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity.Science 244, 331–334.
Kirby, K. S. (1956) A new method for the isolation of nucleic acids from mammalian tissues.Biochem. J. 64, 405–411.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Weber, K., Bolander, M.E. & Sarkar, G. PIG-B: A homemade monophasic cocktail for the extraction of RNA. Mol Biotechnol 9, 73–77 (1998). https://doi.org/10.1007/BF02752699
Issue Date:
DOI: https://doi.org/10.1007/BF02752699