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ThesacB gene cannot be used as a counter-selectable marker inPasteurella multocida

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Abstract

The use of theBacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains ofPasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that thesacB gene can not be used as a positive selection system inP. multocida.

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Authors and Affiliations

  1. Department of Microbiology, Monash University, 3168, Clayton, VIC, Australia

    B. Helen Jost, Preecha Homchampa, Richard A. Strugnell & Ben Adler

Authors
  1. B. Helen Jost
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  2. Preecha Homchampa
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  3. Richard A. Strugnell
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  4. Ben Adler
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Correspondence to Ben Adler.

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Helen Jost, B., Homchampa, P., Strugnell, R.A. et al. ThesacB gene cannot be used as a counter-selectable marker inPasteurella multocida . Mol Biotechnol 8, 189–191 (1997). https://doi.org/10.1007/BF02752263

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  • DOI: https://doi.org/10.1007/BF02752263

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