Abstract
The subcellular localization of parathyroid hormone (PTH) in the normal human parathyroid glands with particular reference to microwave antigen retrieval was investigated using peroxidase-labeled PTH antibody, immunohistochemical, and immunoelectron microscopic methods. The results revealed that PTH granules existed mainly as pro-PTH on the trans side of Golgi and in the regions adjacent to Golgi apparatus. Only a small proportion of secretory granules were stored near the plasma membrane. Microwave irradiation was essential for the immunodetection of PTH.
As the irradiative time extended from 1 to 30 min, the staining intensity increased, and the subcellular preservation decreased. Microwave irradiation for 15 min (with the sections in citrate buffer) with a power output of 500 W is the most ideal for PTH antigen retrieval, as well as for subcellular preservation.
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References
Arnold A, Kim HG, Gaz RD, Eddy RL, Fukushima Y, Byers MG, Shows TB, Kronenberg HM. Molecular cloning and chromosomal mapping of DNA rearranged with the parathyroid hormone gene in a parathyroid adenoma. J Clin Invest 83:2034–2040, 1989.
Inoue Y, Setoguti T. Immunocytochemical localization of parathormone in the mammalian parathyroid gland using the protein A-gold technique. Cell Tissue Res 243:3–7, 1986.
Cinti S, Sbarbati A, Morroni M, Carboni V, Zancanaro C, Cascio VL, Dickersin GR. Parathyroid glands in primary hyperparathyroidism: an ultrastructural study of 25 cases. J Pathol 167:283–290, 1992.
Kendall CH, Roberts PA, Pringle JH, Iauder I. The expression of parathyroid hormone messenger RNA in normal and abnormal parathyroid tissue. J Pathol 165:111–118, 1991.
Stork PJ, Herteaux C, Frazier R, Kronenburg H, Wolfe HJ. Expression and distribution of parathyroid hormone and parathyroid messenger RNA in pathological conditions of the parathyroid. Lab Invest 60:92A (abstract), 1989.
Cinti S, Colussi G, Minoda E, Dickersin GR. Parathyroid glands in primary hyperpara-thyroidism: an ultrastructural study of 50 cases. Hum Pathol 17:1036–1046, 1986.
Takada M. Immunohistochemical classification and ultrastructural study of hyperparathyroidism. Jpn J Urol 83:1767–1773, 1992.
Leong AS-Y, Milios J. An assessment of the efficacy of the microwave-antigen retrieval procedure on a range of tissue antigens. Appl Immunohistochem 1:267–274, 1993.
Swanson PE. Microwave antigen retrieval in citrate buffer. Lab Med 25:520–522, 1994.
Koji T, Brenner RM. Localization of estrogen receptor messenger ribonucleic acid in Rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labelled oligodeoxynucleotides. Endocrinology 132: 382–392, 1993.
Shibuya M, Utsunomiya H, Osamura Y. Immunohistochemical determination of the proliferating cells with monoclonal antibody MIB-1 on paraffin-embedded section using antigen retrieval method (Japanese). Pathol Clin Med 25:520–522, 1993.
Shi SR, Ket ME, Kalra KL. Antigen retrieval in formalin-fixed paraffin-embedded tissues, an enhancement method for immunostaining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741–748, 1991.
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Utsunomiya, H., Shan, L., Kawano, I. et al. Immunolocalization of parathyroid hormone in human parathyroid glands with special references to microwave antigen retrieval. Endocr Pathol 6, 223–227 (1995). https://doi.org/10.1007/BF02739886
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DOI: https://doi.org/10.1007/BF02739886