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Decreased mtDNA copy number of gastric cancer: a new tumor marker?

  • Original Articles
  • Published:
Chinese Journal of Clinical Oncology

Abstract

Objective

To explore the relationship between mtDNA (mitochondnal DNA) and gastric cancer by comparing the difference of mtDNA copy number in gastric cancers and paracancerous tissues.

Methods

The HV1 (hypervariable region) and HV2 of the mitochondrial D-loop region from 20 cases of gastric cancer and 20 paracancerous tissues were amplified by PCR with βactin serving as a quantitative standard marker. The products were separated by polyacrylamide gel electrophoresis (PAGE) and silver stained in order to compare the difference in mtDNA copy number between gastric cancers and paracancerous tissues. The mtDNA copy number was determined for gastric cancers having various pathological characteristics and the results compared with previous immunohistochemical staining of the tumors.

Results

There was a significantly quantitative difference in HV1, HV2 (standardized with βactin) between gastric cancers and paracancerous tissues (P<0.01 ). The mtDNA copy number was associated with important enzymes in the nucleus such as AKP, cAMP-PDE and cGMP-PDE (P< 0.05), but not associated with tumor histological type and invasive depth (P> 0.05).

Conclusion

The occurrence of gastric cancer was closely associated with decreased mtDNA copy number, which may be a new tumor marker.

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Author information

Authors and Affiliations

  1. Department for High Ranking Officials, First Affiliated Hospital, China Medical University, 110001, Shenyang, China

    Fan Li & Jie Lin

  2. Pathology Department, Second Affiliated Hospital of China Medical University, 110004, Shenyang, China

    Xiaosong Wang

  3. The Fourth Laboratory of Cancer Institute, First Affiliated Hospital of China Medical University, 110001, Shenyang, China

    Chengbo Han

Authors
  1. Fan Li
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  2. Xiaosong Wang
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  3. Chengbo Han
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  4. Jie Lin
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Additional information

This work was supported by the research grants from the National Natural Science Foundation of China (No. 30371607).

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Li, F., Wang, X., Han, C. et al. Decreased mtDNA copy number of gastric cancer: a new tumor marker?. Chin. J. Clin. Oncol. 1, 250–255 (2004). https://doi.org/10.1007/BF02739809

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  • DOI: https://doi.org/10.1007/BF02739809

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