Abstract
Glucagon-like peptide-17–36NH2 (GLP-17–36NH2) is a potent stimulator of insulin secretion, as well as of somatostatin-14 (SS-14) release from the pancreatic and gastric D-cells. To investigate the possible effects of this peptide on release of intestinal somatostatin (SS-28 and SS-14), rat intestinal cultures were treated with 10−12–10−6 M GLP-17–36NH2, as well as with the structurally related peptides, GLP-11–36NH2 and GLP-2. Both forms of GLP-1 stimulated dose-dependent increases in intestinal somatostatin; secretion reached 643±126% of controls (p<0.001) after treatment with 10−6 M GLP-17–36NH2, and 398±76% of controls (p<0.001) after 10−6 M GLP-11–36NH2. Thus, GLP-17–36NH2 was more effective than GLP-11–36NH2 in stimulating secretion of intestinal somatostatin-like immunoreactivity (SLI) (p<0.05). GLP-2 did not affect intestinal somatostatin release. Gel permeation analysis demonstrated that 10−6 M GLP-17–36NH2 stimulated SS-28 by 2.9±0.4-fold and SS-14 by 9.1±3.7-fold, whereas GLP-11–36NH2 exerted equivalent effects (2.8±0.9-fold) on both forms of somatostatin. These findings define a novel biological role for GLP-17–36NH2 in the regulation of intestinal somatostatin secretion, and demonstrate that GLP-11–36NH2 exerts unique biological activities in this system.
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Brubaker, P.L., Efendic, S. & Greenberg, G.R. Truncated and full-length glucagon-like peptide-1 (GLP-1) differentially stimulate intestinal somatostatin release. Endocr 6, 91–95 (1997). https://doi.org/10.1007/BF02738808
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DOI: https://doi.org/10.1007/BF02738808