Abstract
Neuropeptides are central to the regulation of mammalian gender-dependent development and reproduction. Preoptic regulatory factor-2 is a neuropeptide gene that is known to be expressed in rat brain and tesis (1). In the brain, expression is gender-dependent and age-dependent (2, 3). Tissue-specific transcripts are found in the preoptic area (POA) of the hypothalamus and in the testis (1). In order to investigate the effects of reproductive hormone status on expression of porf-2 in the male rat, porf-2 transcripts were studied by Northern blot analysis in intact, hypophysectomized, and castrated rat POA, medial basal hypothalamus (MBH), cerebral cortex (CC), testis, and liver. Castration or hypophysectomy increased levels of the brain-specific 0.84 kb 5′ porf-2 transcript in the POA, but did not affect levels of this transcript in the CC. There was a small decrease in the MBH following castration. Hypophysectomy also resulted in a fourfold increase in the 5′ 1.1 kb testis-specific transcript. The affected transcripts are localized to the cytoplasm. A nontissue specific 3′ transcript was also detected. Interestingly, this 0.6 kb transcript became nondetectable in all tissues examined following hypophysectomy. Porf-2 mRNA was also detected in human hypothalamus, testis, adrenal, placenta, and prostate with unique transcripts in each tissue examined. It has been shown elsewhere that porf-2 is a unique single copy gene in the rat genome. These data demonstrate that expression of the porf-2 gene is differentially regulated at the pretranslational level by intrinsic tissue-specific, as well as extrinsic pituitary and gonadal factors. The selected responses to reproductive hormonal status suggest that porf-2 may play a role in hypothalamic pituitary-gonadal interactions.
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References
Nowak, F. V. (1990).Mol. Endocrinol. 4, 1205–1210.
Hu, S. B. and Nowak, F. V. (1994).Mol. Cell. Neurosci. 5, 376–381.
Hu, S. B. and Nowak, F. V. (1995).Endocrine 3, 421–424.
Blum, M. (1989).Methods Enzymol. 168, 618–633.
Sanger, F., Nicklen, S., and Coulson, A. R. (1977).Proc. Natl. Acad. Sci. USA 74, 5463–5467.
Chen, E. Y. and Seeburg, P. H. (1985).DNA 4, 165–170.
Thomas, P. (1983).Methods Enzymol. 100, 255–266.
Church, G. M. and Gilbert W. (1984).Proc. Natl. Acad. Sci. USA 81, 1991–1995.
Breitbart, R. E., Andreadis, A., and Nadal-Ginard, B. (1987).Ann. Rev. Biochem. 56, 467–495.
Emeson, R. B., Hedjran, F., Yeakley, J. M., Guise, J. W., and Rosenfeld, M. G. (1989).Nature 341, 76–80.
DeBold, C. R., Menefee, J. K., Nicholson, W. E., and Orth, D. N. (1988).Mol. Endocrinol. 2, 862–870.
Kilpatrick, D. L., Borland, K., and Jin, D. F. (1987).Proc. Natl. Acad. Sci. USA 84, 5695–5699.
Girardet, C., Walker, W. H., and Habener, J. F. (1996).Mol. Endocrinol. 10, 879–891.
Danoff, A. and Shields, D. (1988).J. Biol. Chem. 263, 16,461–16,466.
Kruys, V., Wathelet, M., Poupart, P., Contreras, R., Fiers, W., Content, J., and Huez, G. (1987).Proc. Natl. Acad. Sci. USA 84, 6030–6034.
Garrett, J. E., Collard, M. W., and Douglass, J. O. (1989).Mol. Cell. Biol. 9, 4381–4389.
Shaw, G. and Kamen, R. (1986).Cell,46, 659–667.
Lowe, W. L. Jr., Roberts, C. T. Jr., Lasky, S. R., and LeRoith, D. (1987).Proc. Natl. Acad. Sci. USA 84, 8946–8950.
Beato, M. (1989).Cell 56, 335–344.
Radovick, S., Ticknor, C. M., Nakayama, Y., Notides, A. C., Rahman, A., Weintraub, B. D., Cutler, G. B., and Wondisford, F. E. (1991).J. Clin. Invest. 88, 1649–1655.
Brann, D. W., McDonald, J. K., Putnam, C. D., and Mahesh, V. B., (1991).Neuroendocrinology 54, 425–432.
Minth-Worby, C. (1994).J. Biol. Chem. 269, 15,460–15,468.
Sahu, A., Phelps, C. P., White, J. D., Crowley, W. R., Kalra, S. P., and Kalra, P. S. (1992).Endocrinology 130, 3331–3336.
Garcia de Yebenes, E., Li, S., Fournier, A., St. Pierre, S., and Pelletier, G. (1995).Brain Res. 674, 112–116.
Wight, P. A., Crew, M. D., and Spindler, S. R. (1987).J. Biol. Chem. 262, 5659–5663.
Nowak, F. V. (1991).Mol. Cell. Neurosci. 2, 395–401.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02738968.
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Nowak, F.V. Modulation of preoptic regulatory factor-2 (porf-2) mRNAs by castration and hypophysectomy. Endocr 6, 57–63 (1997). https://doi.org/10.1007/BF02738803
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DOI: https://doi.org/10.1007/BF02738803