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Effects of quinones on NADH-dependent enzymatic bioluminescent systems

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Abstract

The effects of a number of quinones on the bioluminescence characteristics of a three-component enzymatic system containing alcohol dehydrogenase, bacterial luciferase, and NADH-FMN oxidoreductase were studied to find the most sensitive kinetic parameters of the system intended to be used in biological testing. Both direct and back reactions catalyzed by alcohol dehydrogenase were studied in the presence and in the absence of quinones. The kinetic parameters of the bioluminescent system were found to depend on the redox potentials and concentrations of quinones. The quinone-induced effects were shown to be associated with changes in the NAD+/NADH ratio in the chain of NADH-dependent enzymes. The three-enzyme system based on alcohol dehydrogenase is suggested as a bioluminescence test for ecological monitoring of waste water.

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Abbreviations

ADH:

alcohol dehydrogenase

L:

bacterial luciferase

FMN:

flavine mononucleotide

R:

NADH-FMN oxidoreductase

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Authors and Affiliations

  1. Institute of Biophysics, Siberian Division, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia

    N. S. Kudryasheva, E. N. Esimbekova, I. Yu. Kudinova & V. A. Kratasyuk

  2. Irkutsk State University, Irkutsk, Russia

    D. I. Stom

Authors
  1. N. S. Kudryasheva
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  2. E. N. Esimbekova
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  3. I. Yu. Kudinova
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  4. V. A. Kratasyuk
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  5. D. I. Stom
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Kudryasheva, N.S., Esimbekova, E.N., Kudinova, I.Y. et al. Effects of quinones on NADH-dependent enzymatic bioluminescent systems. Appl Biochem Microbiol 36, 409–413 (2000). https://doi.org/10.1007/BF02738052

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  • DOI: https://doi.org/10.1007/BF02738052

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